biostudies-arrayexpressPekka RappuMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-9497To study the potential role of embigin in the regulation of cellular processes, we used specific siRNAs to knockdown embigin expression in mouse keratinocytes. Two independent siRNAs, Mm_Emb1 and Mm_Emb4 siRNAs, were selected for experiments.After embigin silencing, the cells were harvested and their gene expression patterns were analyzed by RNA sequencing.biostudies-arrayexpressLibrary Construction - Library preparation was performed according to TruSeq Stranded mRNA Sample Preparation protocol (Illumina, 15031047)and TruSeq Stranded mRNA HT Kit (Illumina)Sequencing - Sequencing run was performed using Illumina NovaSeq 6000 SP instrument.Nucleic Acid Extraction - Total RNA was isolated by using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s instructions.Sample Treatment - Subconfluent mouse keratinocytes were transfected with 75 nM siRNA (Mm_Emb_1 and Mm_Emb_4 Flexitube siRNAs and AllStars Negative Control siRNA from QIAGEN) by using siLentFect reagent (Bio-Rad) according to manufacturer’s instructions and cultured for 48 h.Sample Collection - Mouse keratinocytes isolated from adult dorsal skin were cultured in the comprised FAD medium: three parts DMEM medium (Lonza), one part Ham’s F12 medium (Thermo Fisher Scientific) supplemented with 10% FCS (Biowest), 2 mM L-glutamine (Lonza), 10 units/ml Pen-Strep (Lonza), 200 µM adenine (Sigma-Aldrich), 0.5μg/ml hydrocortisone (Sigma-Aldrich), 5μg/ml insulin (Sigma-Aldrich), 16,8 ng/ml cholera toxin (Sigma-Aldrich) and 10 ng/ml epidermal growth factor (Sigma-Aldrich. The cells were cultured at 37°C in a humidified atmosphere with 5% CO2.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - The reads were aligned against the reference genome (Mus musculus GRCm38.95, available in Chipster) using STAR, version 2.7.3 The reads associated with each gene were counted using the HTSeq package, version 0.12.4.Data Transformation - The edgeR R/Bioconductor package was used to normalize gene-wise read counts by TMM normalization method and to perform statistical tests between groups.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusPekka RappuJyrki HeinofalseRNA-seq of mouse keratinocytes treated with siRNA against embiginTo study the potential role of embigin in the regulation of cellular processes, we used specific siRNAs to knockdown embigin expression in mouse keratinocytes. Two independent siRNAs, Mm_Emb1 and Mm_Emb4 siRNAs, were selected for experiments.After embigin silencing, the cells were harvested and their gene expression patterns were analyzed by RNA sequencing.2026-02-10T00:00:00Z2026-02-10T06:42:12.771Z2022-02-04T04:59:18.016ZE-MTAB-9497ERP123682EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969