biostudies-arrayexpressJanos DemeterSalmonella enterica serovar Typhimuriumhttps://www.ebi.ac.uk/biostudies/studies/E-SMDB-3619This experiment set includes all the input and spleen pooled arrays from the first negative selection passaged through 129SvJ mice and represents the 28-day timepoint. "P.2 input" is the same thing as the P.1 output while "P.2 output" represents a sample of the 28-day output repassaged through mice for an additional 28 days. Analysis demonstrated that there was no significant difference between the P.1 and P.2 re-passaged 28-day samples so they are treated the same.biostudies-arrayexpressMIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsFeature Extraction - Title: Feature Extraction. Description: Software and parameters for feature extraction.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsA microarray-based negative selection screen was performed to identify Salmonella enterica serovar Typhimurium (serovar Typhimurium) genes that contribute to long-term systemic infection in 129X1/SvJ (Nramp1(r)) mice. A high-complexity transposon-mutagenized library was used to infect mice intraperitoneally, and the selective disappearance of mutants was monitored after 7, 14, 21, and 28 d postinfection. One hundred and eighteen genes were identified to contribute to serovar Typhimurium infection of the spleens of mice by 28 d postinfection. The negatively selected mutants represent many known aspects of Salmonella physiology and pathogenesis, although the majority of the identified genes are of putative or unknown function. Approximately 30% of the negatively selected genes correspond to transcription profiling by arraySalmonella enterica serovar TyphimuriumGenome-Wide Screen for Salmonella Genes Required for Long-Term Systemic Infection of the Mouse.Janos DemeterKaman ChanTrevor D. Lawley, Kaman Chan, Lucinda J. Thompson, Charles C. Kim, Gregory R. Govoni, Denise M. MonackfalseTranscription profiling of Salmonella passaged through 129SvJ mice to identify genes required for long term systemic infectionThis experiment set includes all the input and spleen pooled arrays from the first negative selection passaged through 129SvJ mice and represents the 28-day timepoint. "P.2 input" is the same thing as the P.1 output while "P.2 output" represents a sample of the 28-day output repassaged through mice for an additional 28 days. Analysis demonstrated that there was no significant difference between the P.1 and P.2 re-passaged 28-day samples so they are treated the same.2006-06-06T00:00:00Z2022-02-04T01:23:22.148Z2022-02-04T01:23:22.148ZE-SMDB-361916518469EFO_000276816518469