biostudies-arrayexpressCarolyn de GraafMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-TABM-371Comparison of Gene Expression in Wild type to Erg Mld2/+ Lin-Sca1+Kit+ cellsbiostudies-arrayexpressNucleic Acid Extraction - Qiagen MicroRNeasy kit as per manufacturer's instructionsLabeling - As per Illumina protocolHybridization - As per Illumina protocolMIAME ScoreOrganizationAssays and DataMAGE-TAB FilesArray DesignsFeature Extraction - As per Illumina protocolAssay Data Transformation - Variance Stabilization Transformation as implemented in the Lumi package for RMetabolomicsUnknownTranscriptomicsGenomicsProteomicsEts-related gene (ERG), which encodes a member of the Ets family of transcription factors, is a potent oncogene. Chromosomal rearrangements involving ERG are found in acute myeloid leukemia, acute lymphoblastic leukemia, Ewing's sarcoma and more than half of all prostate cancers; however, the normal physiological function of Erg is unknown. We did a sensitized genetic screen of the mouse for regulators of hematopoietic stem cell function and report here a germline mutation of Erg. We show that Erg is required for definitive hematopoiesis, adult hematopoietic stem cell function and the maintenance of normal peripheral blood platelet numbers.transcription profiling by arrayMus musculusThe transcription factor Erg is essential for definitive hematopoiesis and the function of adult hematopoietic stem cells.Carolyn de GraafLoughran SJ, Kruse EA, Hacking DF, de Graaf CA, Hyland CD, Willson TA, Henley KJ, Ellis S, Voss AK, Metcalf D, Hilton DJ, Alexander WS, Kile BTfalseTranscription profiling of mouse wild type and Erg Mld2/+ hematopoietic stem cellsComparison of Gene Expression in Wild type to Erg Mld2/+ Lin-Sca1+Kit+ cells2008-07-01T00:00:00Z2022-03-14T19:08:11.799Z2022-03-14T19:08:11.799ZE-TABM-37118500345EFO_000276818500345