{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Anita Grigoriadis"],"disease":["carcinoma"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-TABM-610"],"description":["We describe a novel approach combining RNAi screening in multiple cell lines with expression profiling to identify novel cancer targets in breast cancer cell lines"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - Biotin-labeled cRNA was produced by means of a linear amplification kit (IL1791; Ambion, Austin, TX, http://www.ambion.com) using 250 ng of quality-checked total RNA as input","Nucleic Acid Extraction - RNA was extracted from cell lines with Trizol and phenol/chloroform extraction followed by isopropanol precipitation","Hybridization - Chip hybridisations, washing, Cy3-streptavidin (Amersham Biosciences) staining, and scanning were performed on an Illumina BeadStation 500 (San Diego, http://www.illumina.com) platform using reagents and following protocols supplied by the manufacturer."],"figure_sub":["MIAME Score","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Feature Extraction - Scanning were performed on an Illumina BeadStation 500"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"study_type":["transcription profiling by array"],"species":["Homo sapiens"],"pubmed_title":["Integrated functional, gene expression and genomic analysis for the identification of cancer targets"],"pubmed_authors":["Elizabeth Iorns, Christopher J. Lord, Anita Grigoriadis, Sarah McDonald, Kerry Fenwick, Alan Mackay, Charles A. Mein, Rachael Natrajan, Kay Savage, Narinder Tamber, Jorge S Reis-Filho, Nicholas C. Turner and Alan Ashworth","Anita Grigoriadis"],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling of human cancer cell lines","description":"We describe a novel approach combining RNAi screening in multiple cell lines with expression profiling to identify novel cancer targets in breast cancer cell lines","dates":{"release":"2010-03-02T00:00:00Z","modification":"2021-10-04T12:11:39Z","creation":"2021-10-04T12:11:39Z"},"accession":"E-TABM-610","cross_references":{"EFO":["EFO_0002768"]}}