biostudies-arrayexpressAnita GrigoriadiscarcinomaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-TABM-610We describe a novel approach combining RNAi screening in multiple cell lines with expression profiling to identify novel cancer targets in breast cancer cell linesbiostudies-arrayexpressLabeling - Biotin-labeled cRNA was produced by means of a linear amplification kit (IL1791; Ambion, Austin, TX, http://www.ambion.com) using 250 ng of quality-checked total RNA as inputNucleic Acid Extraction - RNA was extracted from cell lines with Trizol and phenol/chloroform extraction followed by isopropanol precipitationHybridization - Chip hybridisations, washing, Cy3-streptavidin (Amersham Biosciences) staining, and scanning were performed on an Illumina BeadStation 500 (San Diego, http://www.illumina.com) platform using reagents and following protocols supplied by the manufacturer.MIAME ScoreOrganizationAssays and DataMAGE-TAB FilesArray DesignsFeature Extraction - Scanning were performed on an Illumina BeadStation 500UnknownTranscriptomicsGenomicsProteomicstranscription profiling by arrayHomo sapiensIntegrated functional, gene expression and genomic analysis for the identification of cancer targetsElizabeth Iorns, Christopher J. Lord, Anita Grigoriadis, Sarah McDonald, Kerry Fenwick, Alan Mackay, Charles A. Mein, Rachael Natrajan, Kay Savage, Narinder Tamber, Jorge S Reis-Filho, Nicholas C. Turner and Alan AshworthAnita GrigoriadisfalseTranscription profiling of human cancer cell linesWe describe a novel approach combining RNAi screening in multiple cell lines with expression profiling to identify novel cancer targets in breast cancer cell lines2010-03-02T00:00:00Z2021-10-04T12:11:39Z2021-10-04T12:11:39ZE-TABM-610EFO_0002768