biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsJiangning LiLiver regeneration after two-thirds partial hepatectomy (2/3 PH) results in synchronized proliferation of hepatocytes and rapid restoration of liver mass. Understanding the mechanisms that regulate this process has both biological and clinical importance. Using cDNA microarray analysis, we investigated whether gene activation after 2/3 PH is specifically related to liver growth and hepatocyte proliferation. We generated gene expression profiles at 4, 12, 20, and 30 hours after 2/3 PH and compared them with profiles obtained at the same time points after 1/3 PH, a procedure that causes minimal DNA replication. Surprisingly, a significant number of genes whose expression is altered after 2/3 PH are similarly up- or down-regulated after 1/3 PH, particularly at 4 hours. We identified a number transcription profiling by arrayMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-TABM-756biostudies-arrayexpressRelationships between deficits in tissue mass and transcriptional programs after partial hepatectomy in miceGrowth Protocol - Ten-week-old male wild-type C57BL/6 mice (Jackson Laboratory, Bar Harbor, ME) were kept on a 12 hours light/dark cycle with free access to food and water.Hybridization - First we dry down the probe into 3-5 ul using a Speed Vac concentrator. Then we add 40 ul of preheated (42-55?? C) hybridization solution to both sample (Cy3 and Cy5) and mix together. The hybridization mixtures are deposited at one end of the array slidLabeling - cDNA microarrays containing 13,425 mouse cDNAs from the NIA 15K collection. We used 2 arrays for each animal - one array had the Cy3/Cy5 labels associated with L/R probes while the other was a dye reversal (i.e. Cy3/Cy5 R/L). L: sample from left lobe;Sample Processing - A one third partial hepatectomy was done on a mouse liver (left lobe resected). After the elapsed time, the animal was sacraficed and the remaining liver removed. The right lobe was kept.Nucleic Acid Extraction - Total RNA was extracted from a matched pair of frozen liver tissues using Trizol reagent (Invitrogen, Carlsbad, CA) following the manufacturers directions. All RNA was quantified, assessed for quality, and amplified with a single round of T7 amplificati.MIAME ScoreOrganizationAssays and DataMAGE-TAB FilesArray DesignsLi J, Campbell JS, Mitchell C, McMahan RS, Yu X, Riehle KJ, Bumgarner RE, Fausto NJiangning LifalseTranscription profiling of mouse liver after partial hepatectomy in time course2009-09-23T00:00:00Z2021-10-04T17:48:44Z2021-10-04T17:48:44ZE-TABM-75619700759EFO_000276819700759