{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Stefano Volinia"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-TABM-970"],"description":["Normal Tissues"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - Refer to Liu et al (PNAS 101: 9740-9744 (2004)). 5 ug of total RNA was labeled in reverse transcription at 37C for 90 mins by using biotin-labeled rand-octomer oligo primer. The RT reaction mix was further denatured by using 0.5 N NaOH / 1 mM EDTA at 65C for 15 mins and neutralized by 1 M Tris HCl pH 7.6. Biotin signal was detected with an Alexa 647-Streptavidin conjugate.","Nucleic Acid Extraction - RNA was extracted using standard TriZol protocol.","Hybridization - The chips were hybridized on Tecan HS 4800 hybridization station. Chips were pre-hybridized at 25C for 30 min in the buffer of 6x SSPE/ 30% formamide/ 1x Denhardt's solution. The chips were further hybridized with labeled target in 6x SSPE/ 30% formamide at 25C for 18 hr. Hybridization, post-hybridization washing in 0.75x TNT (Tris, sodium, Tween 20) (see PNAS) at 37C for 40 mins. The chips were stained by streptavidin-alexa647 (1:500) dilution in TNT for 30 mins. Post-staining washing in 1x TNT FOR 40 min. Rinse with water and spin dry."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Additional Files","MAGE-TAB Files","Array Designs"],"data_protocol":["Feature Extraction - Arrays were scanned on the GenePix scanner and analyzed using the GenePix Pro software package."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"study_type":["microRNA profiling by array"],"species":["Homo sapiens"],"pubmed_title":["Reprogramming of miRNA networks in cancer and leukemia"],"pubmed_authors":["Stefano Volinia, Marco Galasso, Stefan Costinean, Luca Tagliavini, Giacomo Gamberoni, Alessandra Drusco, Jlenia Marchesini, Nicoletta Mascellani, Maria Elena Sana, Ramzey Abu Jarour, Caroline Desponts, Michael Teitell, Raffaele Baffa, Rami Aqeilan, Marilena V Iorio, Cristian Taccioli, Ramiro Garzon, Gianpiero Di Leva, Muller Fabbri, Marco Catozzi, Maurizio Previati, Stefan Ambs, Tiziana Palumbo, Michela Garofalo, Angelo Veronese, Arianna Bottoni, Pierluigi Gasparini, , Curtis C Harris, Rosa Visone, Yuri Pekarsky, Albert de la Chapelle, Mark Bloomston, Mary Dillhoff, Laura Z Rassenti, Thomas J Kipps, Kay Huebner, Flavia Pichiorri, Dido Lenze, Stefano Cairo, Marie-Annick Buendia, Pascal Pineau, Anne Dejean, Nicola Zanesi, Simona Rossi, George A Calin, Chang-Gong Liu, Jeff Palatini, Massimo Negrini, Andrea Vecchione, Anne Rosenberg & Carlo M Croce.","Stefano Volinia"],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling by array of human normal tissues","description":"Normal Tissues","dates":{"release":"2010-05-18T00:00:00Z","modification":"2022-11-23T07:33:37.523Z","creation":"2022-03-10T01:34:32.343Z"},"accession":"E-TABM-970","cross_references":{"EFO":["EFO_0000753"]}}