biostudies-arrayexpressILSI HESIRattus norvegicusMicroArraySuite 5.0https://www.ebi.ac.uk/biostudies/studies/E-TOXM-39BACKGROUND: The use of gene expression profiling in both clinical and laboratory settings would be enhanced by better characterization of variance due to individual, environmental, and technical factors. Meta-analysis of microarray data from untreated or vehicle-treated animals within the control arm of toxicogenomics studies could yield useful information on baseline fluctuations in gene expression, although control animal data has not been available on a scale and in a form best served for data-mining. RESULTS: A dataset of control animal microarray expression data was assembled by a working group of the Health and Environmental Sciences Institute's Technical Committee on the Application of Genomics in Mechanism Based Risk Assessmenbiostudies-arrayexpressNucleic Acid Extraction - Nucleic acid extraction carried out using Triazole reagentLabeling - Labeling reaction carried out using IVT labeling kit according to manufacturer's recommandationsLabeling - Labeling reaction carried out using other methods, for which no information was availableSample Processing - Animal handling carried out in accordance with ethical guidelines.Labeling - Labeling reaction carried out using ENZO Kit according to manufacturer's recommandationsNucleic Acid Extraction - Nucleic acid extraction carried out using Qiagen RNeasy KitNucleic Acid Extraction - Nucleic acid extraction carried out using other methodHybridization - Hybridization carried out according to Manufacturer's recommandationsMIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsFeature Extraction - Title: Affymetrix CEL analysis. Description:MetabolomicsUnknownTranscriptomicsGenomicsProteomicsRattus norvegicusSources of variation in baseline gene expression levels from toxicogenomics study control animals across multiple laboratoriesMichael J. Boedigheimer, Russell D. Wolfinger, Michael B. Bass, Pierre R. Bushel, Jeff W. Chou, Matthew Cooper, J. Christopher Corton, Jennifer Fostel, Susan Hester, Janice S. Lee, Fenglong Liu, Jie Liu, Hui-Rong Qian, John Quackenbush, Syril Pettit, and Karol L. ThompsonILSI HESIfalseTranscription profiling of rat liver and kidney samples performed in 16 different institutions to determine study factors which are key sources of variability. Raw files available as additional archives.BACKGROUND: The use of gene expression profiling in both clinical and laboratory settings would be enhanced by better characterization of variance due to individual, environmental, and technical factors. Meta-analysis of microarray data from untreated or vehicle-treated animals within the control arm of toxicogenomics studies could yield useful information on baseline fluctuations in gene expression, although control animal data has not been available on a scale and in a form best served for data-mining. RESULTS: A dataset of control animal microarray expression data was assembled by a working group of the Health and Environmental Sciences Institute's Technical Committee on the Application of Genomics in Mechanism Based Risk Assessmen2008-10-10T00:00:00Z2022-03-10T00:53:44.814Z2022-03-10T00:53:44.814ZE-TOXM-39