{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Branko Braam"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-UMCU-9"],"description":["Control samples of HUVEC were obtained parallel with a 4 hr experiment and compared to control."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Huvec cells were harvested from freshly obtained umbilical cords."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["Shear stress modulates gene expression in endothelial cells (ECs) partly through nitric oxide (NO), acting via enhanced cGMP formation by guanylyl cyclase (GC). We addressed non-cGMP-mediated transcriptional responses to shear stress in human umbilical ECs subjected to high-laminar shear stress (25 dyn/cm2; 150 minutes). RNA was isolated, reverse-transcribed, Cy3/5-labeled, and hybridized to 19 K human microarrays. High shear (n=6), high shear with 100 micromol/L L-NAME (n=3), and high shear with 10 micromol/L ODQ (GC inhibitor) in the perfusate (n=3) was compared with samples not subjected to flow. Among genes responding to high shear were HMOX1 (up) and PPARG (down). A high percentage of gene expression modulation by shear was absent during concomitant L-NAME or ODQ. Several transcriptio"],"study_type":["transcription profiling by array"],"species":["Homo sapiens"],"pubmed_title":["Nitric oxide-dependent and nitric oxide-independent transcriptional responses to high shear stress in endothelial cells"],"pubmed_authors":["Patrick Kemmeren","Braam B, de Roos R, Bluyssen H, Kemmeren P, Holstege FCP, Joles JA, Koomans H","Frank Holstege","Remmert de Roos","Hans Bluysen","Hein Koomans","Branko Braam","Adele Dijk"],"additional_accession":[]},"is_claimable":false,"name":"Control over Control","description":"Control samples of HUVEC were obtained parallel with a 4 hr experiment and compared to control.","dates":{"release":"2005-04-01T00:00:00Z","modification":"2022-02-03T08:59:59.518Z","creation":"2022-02-03T08:59:59.518Z"},"accession":"E-UMCU-9","cross_references":{"pubmed":["15699468"],"EFO":["EFO_0002768"],"doi":["15699468"]}}