biostudies-arrayexpressBranko BraamHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-UMCU-9Control samples of HUVEC were obtained parallel with a 4 hr experiment and compared to control.biostudies-arrayexpressGrowth Protocol - Huvec cells were harvested from freshly obtained umbilical cords.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsMetabolomicsUnknownTranscriptomicsGenomicsProteomicsShear stress modulates gene expression in endothelial cells (ECs) partly through nitric oxide (NO), acting via enhanced cGMP formation by guanylyl cyclase (GC). We addressed non-cGMP-mediated transcriptional responses to shear stress in human umbilical ECs subjected to high-laminar shear stress (25 dyn/cm2; 150 minutes). RNA was isolated, reverse-transcribed, Cy3/5-labeled, and hybridized to 19 K human microarrays. High shear (n=6), high shear with 100 micromol/L L-NAME (n=3), and high shear with 10 micromol/L ODQ (GC inhibitor) in the perfusate (n=3) was compared with samples not subjected to flow. Among genes responding to high shear were HMOX1 (up) and PPARG (down). A high percentage of gene expression modulation by shear was absent during concomitant L-NAME or ODQ. Several transcriptiotranscription profiling by arrayHomo sapiensNitric oxide-dependent and nitric oxide-independent transcriptional responses to high shear stress in endothelial cellsPatrick KemmerenBraam B, de Roos R, Bluyssen H, Kemmeren P, Holstege FCP, Joles JA, Koomans HFrank HolstegeRemmert de RoosHans BluysenHein KoomansBranko BraamAdele DijkfalseControl over ControlControl samples of HUVEC were obtained parallel with a 4 hr experiment and compared to control.2005-04-01T00:00:00Z2022-02-03T08:59:59.518Z2022-02-03T08:59:59.518ZE-UMCU-915699468EFO_000276815699468