biostudies-literatureLiu CNatural Science Foundation of ChinaResearch Funds for the Central UniversitiesNational Key Research and Development Program of China1610https://www.ebi.ac.uk/biostudies/studies/S-EPMC10000504biostudies-literatureUnknown15(5)<h4>Background</h4>Gastric cancer is a malignant tumor with high morbidity and mortality. Therefore, the accurate recognition of prognostic molecular markers is the key to improving treatment efficacy and prognosis.<h4>Methods</h4>In this study, we developed a stable and robust signature through a series of processes using machine-learning approaches. This PRGS was further experimentally validated in clinical samples and a gastric cancer cell line.<h4>Results</h4>The PRGS is an independent risk factor for overall survival that performs reliably and has a robust utility. Notably, PRGS proteins promote cancer cell proliferation by regulating the cell cycle. Besides, the high-risk group displayed a lower tumor purity, higher immune cell infiltration, and lower oncogenic mutation than the low-PRGS group.<h4>Conclusions</h4>This PRGS could be a powerful and robust tool to improve clinical outcomes for individual gastric cancer patients.CancersDevelopment and Experimental Validation of a Novel Prognostic Signature for Gastric Cancer.PMC100005042019RC045620030282017YFA0505503Li XYin FShi MHuo YZhang YGao JLiu CJin PWang ZZhang MQChen TfalseDevelopment and Experimental Validation of a Novel Prognostic Signature for Gastric Cancer.<h4>Background</h4>Gastric cancer is a malignant tumor with high morbidity and mortality. Therefore, the accurate recognition of prognostic molecular markers is the key to improving treatment efficacy and prognosis.<h4>Methods</h4>In this study, we developed a stable and robust signature through a series of processes using machine-learning approaches. This PRGS was further experimentally validated in clinical samples and a gastric cancer cell line.<h4>Results</h4>The PRGS is an independent risk factor for overall survival that performs reliably and has a robust utility. Notably, PRGS proteins promote cancer cell proliferation by regulating the cell cycle. Besides, the high-risk group displayed a lower tumor purity, higher immune cell infiltration, and lower oncogenic mutation than the low-PRGS group.<h4>Conclusions</h4>This PRGS could be a powerful and robust tool to improve clinical outcomes for individual gastric cancer patients.2023-01-01T00:00:00Z2023 Mar2024-12-03T21:24:12.089Z2024-12-03T21:24:12.089ZS-EPMC100005043690040110.3390/cancers15051610