{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ang AKR"],"funding":["Japan Society for the Promotion of Science"],"pagination":["368-378"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10000799"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30(Pt 2)"],"pubmed_abstract":["X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurem"],"journal":["Journal of synchrotron radiation"],"pubmed_title":["Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals."],"pmcid":["PMC10000799"],"funding_grant_id":["26105006","20H05881","JP17H06372","20H05883","20H05884","26105005","17H05219","20H05878","JP16KT0058"],"pubmed_authors":["Happo N","Kawamura N","Marumi R","Ang AKR","Kimura K","Umena Y","Hayashi K","Matsushita T","Shen JR","Sato-Tomita A","Yamamoto Y","Sasaki YC","Shibayama N","Takano Y"],"additional_accession":[]},"is_claimable":false,"name":"Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals.","description":"X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurem","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-04T03:35:31.882Z","creation":"2025-04-04T03:35:31.882Z"},"accession":"S-EPMC10000799","cross_references":{"pubmed":["36891850"],"doi":["10.1107/S1600577522011833"]}}