{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["207"],"submitter":["Vidal LEL"],"funding":["Finep"],"pubmed_abstract":["COVID-19 pandemic was caused by the severe acute respiratory syndrome coronavirus 2 (Sars-CoV-2). The nucleocapsid (N) protein from Sars-CoV-2 is a highly immunogenic antigen and responsible for genome packing. Serological assays are important tools to detect previous exposure to SARS-CoV-2, complement epidemiological studies, vaccine evaluation and also in COVID-19 surveillance. SARS-CoV-2 N (r2N) protein was produced in Escherichia coli, characterized, and the immunological performance was evaluated by enzyme-linked immunosorbent assay (ELISA) and beads-based array immunoassay. r2N protein oligomers were evidenced when it is associated to nucleic acid. Benzonase treatment reduced host nucleic acid associated to r2N protein, but crosslinking assay still demonstrates the presence of higher"],"journal":["Protein expression and purification"],"pagination":["106263"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10012136"],"repository":["biostudies-literature"],"pubmed_title":["Process development and characterization of recombinant nucleocapsid protein for its application on COVID-19 diagnosis."],"pmcid":["PMC10012136"],"pubmed_authors":["Vidal LEL","Miguez M","de Mello MB","Pestana CP","Loureiro BO","Bastos RC","Figueira-Mansur J","Argondizzo APC","Marques CFS","da Silva LBR","Trinta KS","Jurgilas PB","da Silva Junior HC","Martins FO","da Silva ED","Esteves G"],"additional_accession":[]},"is_claimable":false,"name":"Process development and characterization of recombinant nucleocapsid protein for its application on COVID-19 diagnosis.","description":"COVID-19 pandemic was caused by the severe acute respiratory syndrome coronavirus 2 (Sars-CoV-2). The nucleocapsid (N) protein from Sars-CoV-2 is a highly immunogenic antigen and responsible for genome packing. Serological assays are important tools to detect previous exposure to SARS-CoV-2, complement epidemiological studies, vaccine evaluation and also in COVID-19 surveillance. SARS-CoV-2 N (r2N) protein was produced in Escherichia coli, characterized, and the immunological performance was evaluated by enzyme-linked immunosorbent assay (ELISA) and beads-based array immunoassay. r2N protein oligomers were evidenced when it is associated to nucleic acid. Benzonase treatment reduced host nucleic acid associated to r2N protein, but crosslinking assay still demonstrates the presence of higher","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-27T02:24:56.407Z","creation":"2024-11-20T11:05:00.947Z"},"accession":"S-EPMC10012136","cross_references":{"pubmed":["36921810"],"doi":["10.1016/j.pep.2023.106263"]}}