biostudies-literatureUnknown120(10)Jiang HThe association of decreased fecundity with insecticide resistance and the negative sublethal effects of insecticides on insect reproduction indicates the typical trade-off between two highly energy-demanding processes, detoxification and reproduction. However, the underlying mechanisms are poorly understood. The energy sensor adenosine monophosphate-activated protein kinase (AMPK) and the transcription factor Cap "n" collar isoform C (CncC) are important regulators of energy metabolism and xenobiotic response, respectively. In this study, using the beetle <i>Tribolium castaneum</i> as a model organism, we found that deltamethrin-induced oxidative stress activated AMPK, which promoted the nuclear translocation of CncC through its phosphorylation. The CncC not only acts as a transcription aProceedings of the National Academy of Sciences of the United States of Americae2214038120https://www.ebi.ac.uk/biostudies/studies/S-EPMC10013871biostudies-literatureThe pleiotropic AMPK-CncC signaling pathway regulates the trade-off between detoxification and reproduction.PMC10013871Zheng YPark YJiang HGe HZhang NWei JQian KWang JMeng XReddy Palli SfalseThe pleiotropic AMPK-CncC signaling pathway regulates the trade-off between detoxification and reproduction.The association of decreased fecundity with insecticide resistance and the negative sublethal effects of insecticides on insect reproduction indicates the typical trade-off between two highly energy-demanding processes, detoxification and reproduction. However, the underlying mechanisms are poorly understood. The energy sensor adenosine monophosphate-activated protein kinase (AMPK) and the transcription factor Cap "n" collar isoform C (CncC) are important regulators of energy metabolism and xenobiotic response, respectively. In this study, using the beetle <i>Tribolium castaneum</i> as a model organism, we found that deltamethrin-induced oxidative stress activated AMPK, which promoted the nuclear translocation of CncC through its phosphorylation. The CncC not only acts as a transcription a2023-01-01T00:00:00Z2023 Mar2025-04-04T20:07:15.543Z2025-04-04T20:07:15.543ZS-EPMC100138713685394610.1073/pnas.2214038120