{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Wittmann N"],"pubmed_abstract":["<h4>Objective</h4>The formation of large intracellular protein aggregates of the inflammasome adaptor ASC is a hallmark of inflammasome activation and characteristic of autoinflammation. Inflammasome activated cells release the highly proinflammatory cytokine IL-1β in addition to ASC specks into the extracellular space. Autoinflammatory activity has been demonstrated in systemic JIA, however minimal data exist on the role of inflammasomes in other JIA subtypes. We therefore investigated, if pyroptotic cells are present in the circulation of oligo- and poly-articular JIA.<h4>Methods</h4>Peripheral blood of JIA patients (<i>n</i> = 46) was investigated for ASC speck formation, a key step in inflammasome activation, by flow cytometry and immunofluorescence. Free ASC and proinflammatory cytoki"],"journal":["Frontiers in medicine"],"pagination":["1063772"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10014801"],"repository":["biostudies-literature"],"pubmed_title":["Inflammasome activation and formation of ASC specks in patients with juvenile idiopathic arthritis."],"pmcid":["PMC10014801"],"pubmed_authors":["Bossaller L","Gramenz J","Meyer-Bahlburg A","Behrendt AK","Wittmann N","Mishra N","Kuthning D"],"additional_accession":[]},"is_claimable":false,"name":"Inflammasome activation and formation of ASC specks in patients with juvenile idiopathic arthritis.","description":"<h4>Objective</h4>The formation of large intracellular protein aggregates of the inflammasome adaptor ASC is a hallmark of inflammasome activation and characteristic of autoinflammation. Inflammasome activated cells release the highly proinflammatory cytokine IL-1β in addition to ASC specks into the extracellular space. Autoinflammatory activity has been demonstrated in systemic JIA, however minimal data exist on the role of inflammasomes in other JIA subtypes. We therefore investigated, if pyroptotic cells are present in the circulation of oligo- and poly-articular JIA.<h4>Methods</h4>Peripheral blood of JIA patients (<i>n</i> = 46) was investigated for ASC speck formation, a key step in inflammasome activation, by flow cytometry and immunofluorescence. Free ASC and proinflammatory cytoki","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-05T16:11:16.122Z","creation":"2025-04-05T16:11:16.122Z"},"accession":"S-EPMC10014801","cross_references":{"pubmed":["36936231"],"doi":["10.3389/fmed.2023.1063772"]}}