biostudies-literatureSanto DFunda??o para a Ci?ncia e a TecnologiaEuropean Regional Development Fund1274-1286https://www.ebi.ac.uk/biostudies/studies/S-EPMC10015461biostudies-literatureUnknown24(3)Cationic glycopolymers stand out as gene delivery nanosystems due to their inherent biocompatibility and high binding affinity to the asialoglycoprotein receptor (ASGPR), a target receptor overexpressed in hepatocellular carcinoma (HCC) cells. However, their synthesis procedure remains laborious and complex, with problems of solubilization and the need for protection/deprotection steps. Here, a mini-library of well-defined poly(2-aminoethyl methacrylate hydrochloride-<i>co</i>-poly(2-lactobionamidoethyl methacrylate) (PAMA-<i>co</i>-PLAMA) glycopolymers was synthesized by activators regenerated by electron transfer (ARGET) ATRP to develop an efficient gene delivery nanosystem. The glycoplexes generated had suitable physicochemical properties and showed high ASGPR specificity and high transBiomacromoleculesGlycopolymers Mediate Suicide Gene Therapy in ASGPR-Expressing Hepatocellular Carcinoma Cells in Tandem with Docetaxel.PMC10015461UIDP/04539/2020RECI/QEQ-QFI/0168/2012REEQ/481/QUI/20062020.00186.CEECINDSFRH/BD/132601/2017UIDB/04539/2020POCI-01-0145-FEDER-30916CENTRO-07-CT62-FEDER-002012IF/01007/2015Santo DSerra ACMendonca PVCoelho JFJFaneca HCordeiro RAfalseGlycopolymers Mediate Suicide Gene Therapy in ASGPR-Expressing Hepatocellular Carcinoma Cells in Tandem with Docetaxel.Cationic glycopolymers stand out as gene delivery nanosystems due to their inherent biocompatibility and high binding affinity to the asialoglycoprotein receptor (ASGPR), a target receptor overexpressed in hepatocellular carcinoma (HCC) cells. However, their synthesis procedure remains laborious and complex, with problems of solubilization and the need for protection/deprotection steps. Here, a mini-library of well-defined poly(2-aminoethyl methacrylate hydrochloride-<i>co</i>-poly(2-lactobionamidoethyl methacrylate) (PAMA-<i>co</i>-PLAMA) glycopolymers was synthesized by activators regenerated by electron transfer (ARGET) ATRP to develop an efficient gene delivery nanosystem. The glycoplexes generated had suitable physicochemical properties and showed high ASGPR specificity and high trans2023-01-01T00:00:00Z2023 Mar2025-04-04T15:01:49.768Z2025-04-04T15:01:49.768ZS-EPMC100154613678031410.1021/acs.biomac.2c01329