biostudies-literatureZath GKDefense Advanced Research Projects AgencyMontana State UniversityDeutsche ForschungsgemeinschaftNIAID NIH HHSNational Institutes of HealthMaterials Research Science and Engineering Center, Harvard UniversityNational Science Foundation4735-4745https://www.ebi.ac.uk/biostudies/studies/S-EPMC10016142biostudies-literatureUnknown22(23)In drop-based microfluidics, an aqueous sample is partitioned into drops using individual pump sources that drive water and oil into a drop-making device. Parallelization of drop-making devices is necessary to achieve high-throughput screening of multiple experimental conditions, especially in time-sensitive studies. Here, we present the plate-interfacing parallel encapsulation (PIPE) chip, a microfluidic chip designed to generate 50 to 90 μm diameter drops of up to 96 different conditions in parallel by interfacing individual drop makers with a standard 384-well microtiter plate. The PIPE chip is used to generate two types of optically barcoded drop libraries consisting of two-color fluorescent particle combinations: a library of 24 microbead barcodes and a library of 192 quantum dot barcLab on a chipRapid parallel generation of a fluorescently barcoded drop library from a microtiter plate using the plate-interfacing parallel encapsulation (PIPE) chip.PMC10016142CAREER DMR-1753352DMR-2011754R21 AI1519231R21Al151923SP 1282/1-1W911NF-17-2-0034Zath GKHoffman CWChang CBBikos DAAbate ARSperling RAAbbasi RWeitz DAfalseRapid parallel generation of a fluorescently barcoded drop library from a microtiter plate using the plate-interfacing parallel encapsulation (PIPE) chip.In drop-based microfluidics, an aqueous sample is partitioned into drops using individual pump sources that drive water and oil into a drop-making device. Parallelization of drop-making devices is necessary to achieve high-throughput screening of multiple experimental conditions, especially in time-sensitive studies. Here, we present the plate-interfacing parallel encapsulation (PIPE) chip, a microfluidic chip designed to generate 50 to 90 μm diameter drops of up to 96 different conditions in parallel by interfacing individual drop makers with a standard 384-well microtiter plate. The PIPE chip is used to generate two types of optically barcoded drop libraries consisting of two-color fluorescent particle combinations: a library of 24 microbead barcodes and a library of 192 quantum dot barc2022-01-01T00:00:00Z2022 Nov2025-04-21T16:13:43.141Z2025-04-21T16:13:43.141ZS-EPMC100161423636713910.1039/d2lc00909a