biostudies-literatureMcKerrow WNIA NIH HHSIDeANIANational Cancer InstituteNCI NIH HHSNIHNIGMS NIH HHSNIGMS2033-2045https://www.ebi.ac.uk/biostudies/studies/S-EPMC10018344biostudies-literatureUnknown51(5)LINE-1 retrotransposons are sequences capable of copying themselves to new genomic loci via an RNA intermediate. New studies implicate LINE-1 in a range of diseases, especially in the context of aging, but without an accurate understanding of where and when LINE-1 is expressed, a full accounting of its role in health and disease is not possible. We therefore developed a method-5' scL1seq-that makes use of a widely available library preparation method (10x Genomics 5' single cell RNA-seq) to measure LINE-1 expression in tens of thousands of single cells. We recapitulated the known pattern of LINE-1 expression in tumors-present in cancer cells, absent from immune cells-and identified hitherto undescribed LINE-1 expression in human epithelial cells and mouse hippocampal neurons. In both cases, we saw a modest increase with age, supporting recent research connecting LINE-1 to age related diseases.Nucleic acids researchLINE-1 retrotransposon expression in cancerous, epithelial and neuronal cells revealed by 5' single-cell RNA-Seq.PMC10018344R21 CA235521P20 GM109035P01 AG051449R01 AG050582HHSN261200800001EP01AG051449U24 CA2109721R01AG050582-01A1U24CA210972P20GM1090351R21CA235521Evans SASedivy JMMcKerrow WNeretti NBoeke JDKagermazova LFrazzette NCarucci JDoudican NRocha AFenyo DKaparos EIfalseLINE-1 retrotransposon expression in cancerous, epithelial and neuronal cells revealed by 5' single-cell RNA-Seq.LINE-1 retrotransposons are sequences capable of copying themselves to new genomic loci via an RNA intermediate. New studies implicate LINE-1 in a range of diseases, especially in the context of aging, but without an accurate understanding of where and when LINE-1 is expressed, a full accounting of its role in health and disease is not possible. We therefore developed a method-5' scL1seq-that makes use of a widely available library preparation method (10x Genomics 5' single cell RNA-seq) to measure LINE-1 expression in tens of thousands of single cells. We recapitulated the known pattern of LINE-1 expression in tumors-present in cancer cells, absent from immune cells-and identified hitherto undescribed LINE-1 expression in human epithelial cells and mouse hippocampal neurons. In both cases, we saw a modest increase with age, supporting recent research connecting LINE-1 to age related diseases.2023-01-01T00:00:00Z2023 Mar2024-10-15T05:11:58.538Z2024-10-15T05:11:58.538ZS-EPMC100183443674443710.1093/nar/gkad049