{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Al Mamun AAM"],"funding":["NIAID NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pagination":["102652"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10018792"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["123-124"],"pubmed_abstract":["Two phylogenetically distantly-related IncF plasmids, F and pED208, serve as important models for mechanistic and structural studies of F-like type IV secretion systems (T4SS<sub>F</sub>s) and F pili. Here, we present the pED208 sequence and compare it to F and pUMNF18, the closest match to pED208 in the NCBI database. As expected, gene content of the three cargo regions varies extensively, although the maintenance/leading regions (MLRs) and transfer (Tra) regions also carry novel genes or motifs with predicted modulatory effects on plasmid stability, dissemination and host range. By use of a Cre recombinase assay for translocation (CRAfT), we recently reported that pED208-carrying donors translocate several products of the MLR (ParA, ParB1, ParB2, SSB, PsiB, PsiA) intercellularly through "],"journal":["Plasmid"],"pubmed_title":["IncFV plasmid pED208: Sequence analysis and evidence for translocation of maintenance/leading region proteins through diverse type IV secretion systems."],"pmcid":["PMC10018792"],"funding_grant_id":["R21 AI159970","R01GM48746","K01 AI148593","R35GM131892","R35 GM131892","R21AI159970","R01 GM048746"],"pubmed_authors":["Hanson B","Shropshire WC","Kissoon K","Christie PJ","Al Mamun AAM","Kishida K"],"additional_accession":[]},"is_claimable":false,"name":"IncFV plasmid pED208: Sequence analysis and evidence for translocation of maintenance/leading region proteins through diverse type IV secretion systems.","description":"Two phylogenetically distantly-related IncF plasmids, F and pED208, serve as important models for mechanistic and structural studies of F-like type IV secretion systems (T4SS<sub>F</sub>s) and F pili. Here, we present the pED208 sequence and compare it to F and pUMNF18, the closest match to pED208 in the NCBI database. As expected, gene content of the three cargo regions varies extensively, although the maintenance/leading regions (MLRs) and transfer (Tra) regions also carry novel genes or motifs with predicted modulatory effects on plasmid stability, dissemination and host range. By use of a Cre recombinase assay for translocation (CRAfT), we recently reported that pED208-carrying donors translocate several products of the MLR (ParA, ParB1, ParB2, SSB, PsiB, PsiA) intercellularly through ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep-Nov","modification":"2026-05-29T19:14:44.237Z","creation":"2024-10-15T05:12:39.87Z"},"accession":"S-EPMC10018792","cross_references":{"pubmed":["36228885"],"doi":["10.1016/j.plasmid.2022.102652"]}}