biostudies-literatureLiu Xthe National Nature Science Foundation of ChinaThe National Nature Science Foundation of China94https://www.ebi.ac.uk/biostudies/studies/S-EPMC10022307biostudies-literatureUnknown21(1)<h4>Background</h4>Currently, the etiology of idiopathic short stature (ISS) is still unclear. The poor understanding of the molecular mechanisms of ISS has largely restricted this strategy towards safe and effective clinical therapies.<h4>Methods</h4>The plasma exosomes of ISS children were co-cultured with normal human chondrocytes. The differential expression of exosome miRNA between ISS and normal children was identified via high-throughput microRNA sequencing and bioinformatics analysis. Immunohistochemistry, In situ hybridization, RT-qPCR, western blotting, luciferase expression, and gene overexpression and knockdown were performed to reveal the key signaling pathways that exosome miRNA of aberrant expression in ISS children impairs longitudinal bone growth.<h4>Results</h4>ChondrocytJournal of nanobiotechnologyPlasma exosome miRNA-26b-3p derived from idiopathic short stature impairs longitudinal bone growth via the AKAP2/ERK1/2 axis.PMC100223078196039282060169Liu XWu ZZhang JShen YYuan JJia JfalsePlasma exosome miRNA-26b-3p derived from idiopathic short stature impairs longitudinal bone growth via the AKAP2/ERK1/2 axis.<h4>Background</h4>Currently, the etiology of idiopathic short stature (ISS) is still unclear. The poor understanding of the molecular mechanisms of ISS has largely restricted this strategy towards safe and effective clinical therapies.<h4>Methods</h4>The plasma exosomes of ISS children were co-cultured with normal human chondrocytes. The differential expression of exosome miRNA between ISS and normal children was identified via high-throughput microRNA sequencing and bioinformatics analysis. Immunohistochemistry, In situ hybridization, RT-qPCR, western blotting, luciferase expression, and gene overexpression and knockdown were performed to reveal the key signaling pathways that exosome miRNA of aberrant expression in ISS children impairs longitudinal bone growth.<h4>Results</h4>Chondrocyt2023-01-01T00:00:00Z2023 Mar2025-04-22T18:24:57.549Z2025-04-06T02:24:30.328ZS-EPMC100223073692777910.1186/s12951-023-01849-8