biostudies-literatureUnknown11Prasertsilp PChulalongkorn UniversityCassava pulp is one of the most abundant agricultural residues that can cause serious disposal problems. This study aimed to apply a biorefinery approach by examining the feasibility of microwave-assisted cassava pulp hydrolysis to attain sustainable management and efficient use of natural resources. Four factors, namely, the liquid-to-solid ratio (20 mL/g, 10 mL/g, 7.5 mL/g, and 5 mL/g), types of acids (H₂SO₄ and H₃PO₄), watt power (600 W, 700 W, and 800 W) and time (3, 5 and 8 min), were carefully investigated. The highest fermentable sugar content of 88.1 g/L ± 0.7 g/L (0.88 g fermentable sugars/g dry cassava pulp) was achieved when 20 mL/g cassava pulp was hydrolyzed with 2.5% (v/v) H₂SO₄ under microwave irradiation at 8Frontiers in bioengineering and biotechnology1131053https://www.ebi.ac.uk/biostudies/studies/S-EPMC10025311biostudies-literatureMicrowave-assisted cassava pulp hydrolysis as food waste biorefinery for biodegradable polyhydroxybutyrate production.PMC10025311Pattaragulwanit KKim BSNapathorn SCPrasertsilp PfalseMicrowave-assisted cassava pulp hydrolysis as food waste biorefinery for biodegradable polyhydroxybutyrate production.Cassava pulp is one of the most abundant agricultural residues that can cause serious disposal problems. This study aimed to apply a biorefinery approach by examining the feasibility of microwave-assisted cassava pulp hydrolysis to attain sustainable management and efficient use of natural resources. Four factors, namely, the liquid-to-solid ratio (20 mL/g, 10 mL/g, 7.5 mL/g, and 5 mL/g), types of acids (H₂SO₄ and H₃PO₄), watt power (600 W, 700 W, and 800 W) and time (3, 5 and 8 min), were carefully investigated. The highest fermentable sugar content of 88.1 g/L ± 0.7 g/L (0.88 g fermentable sugars/g dry cassava pulp) was achieved when 20 mL/g cassava pulp was hydrolyzed with 2.5% (v/v) H₂SO₄ under microwave irradiation at 82023-01-01T00:00:00Z20232025-07-02T03:04:27.653Z2025-07-02T03:04:27.653ZS-EPMC100253113694988410.3389/fbioe.2023.1131053