{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Leyhr J"],"funding":["European Synchrotron Radiation Facility","Vetenskapsrådet","Stiftelsen Lars Hiertas Minne","Stiftelsen Zoologisk Forskning"],"pagination":["1108916"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10025580"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14"],"pubmed_abstract":["Detailed histological analyses are desirable for zebrafish mutants that are models for human skeletal diseases, but traditional histological techniques are limited to two-dimensional thin sections with orientations highly dependent on careful sample preparation. On the other hand, techniques that provide three-dimensional (3D) datasets including µCT scanning are typically limited to visualizing the bony skeleton and lack histological resolution. We combined diffusible iodine-based contrast enhancement (DICE) and propagation phase-contrast synchrotron radiation micro-computed tomography (PPC-SRµCT) to image late larval and juvenile zebrafish, obtaining high-quality 3D virtual histology datasets of the mineralized skeleton and surrounding soft tissues. To demonstrate this technique, we used "],"journal":["Frontiers in endocrinology"],"pubmed_title":["Enhanced contrast synchrotron X-ray microtomography for describing skeleton-associated soft tissue defects in zebrafish mutants."],"pmcid":["PMC10025580"],"funding_grant_id":["2022-04988, 2019-04595","LS3021"],"pubmed_authors":["Haitina T","Dollman KN","Sanchez S","Leyhr J","Tafforeau P"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced contrast synchrotron X-ray microtomography for describing skeleton-associated soft tissue defects in zebrafish mutants.","description":"Detailed histological analyses are desirable for zebrafish mutants that are models for human skeletal diseases, but traditional histological techniques are limited to two-dimensional thin sections with orientations highly dependent on careful sample preparation. On the other hand, techniques that provide three-dimensional (3D) datasets including µCT scanning are typically limited to visualizing the bony skeleton and lack histological resolution. We combined diffusible iodine-based contrast enhancement (DICE) and propagation phase-contrast synchrotron radiation micro-computed tomography (PPC-SRµCT) to image late larval and juvenile zebrafish, obtaining high-quality 3D virtual histology datasets of the mineralized skeleton and surrounding soft tissues. To demonstrate this technique, we used ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-06-25T03:15:19.453Z","creation":"2026-06-25T03:07:52.782Z"},"accession":"S-EPMC10025580","cross_references":{"pubmed":["36950679"],"doi":["10.3389/fendo.2023.1108916"]}}