biostudies-literatureKim HSKorea Health Industry Development InstituteNational Research Foundation of Korea1101291https://www.ebi.ac.uk/biostudies/studies/S-EPMC10028079biostudies-literatureUnknown13<h4>Background & aims</h4><i>Fusobacterium nucleatum</i> (FN) plays a pivotal role in the development and progression of colorectal cancer by modulating antitumor immune responses. However, the impact of FN on immune regulation in the tumor microenvironment has not been fully elucidated.<h4>Methods</h4>The abundance of FN was measured in 99 stage III CRC tumor tissues using quantitative polymerase chain reaction. Gene expression profiles were assessed and annotated using consensus molecular subtypes (CMS), Gene Ontology (GO) analysis, and deconvolution of individual immune cell types in the context of FN abundance. Immune profiling for tumor infiltrating T cells isolated from human tumor tissues was analyzed using flow cytometry. <i>Ex vivo</i> tumor-infiltrating T cells were stimulated inFrontiers in cellular and infection microbiology<i>Fusobacterium nucleatum</i> induces a tumor microenvironment with diminished adaptive immunity against colorectal cancers.PMC100280792022R1A2C4001879, 2022M3A9F3016364, 2022R1I1A1A01072740, 2020R1F1A1066973HI22C0353Kim CGMin BSKim HAhn JBKim HSLee KShin SJLee HPaik SKim WKKim KAShin ECYoo JKim TMfalse<i>Fusobacterium nucleatum</i> induces a tumor microenvironment with diminished adaptive immunity against colorectal cancers.<h4>Background & aims</h4><i>Fusobacterium nucleatum</i> (FN) plays a pivotal role in the development and progression of colorectal cancer by modulating antitumor immune responses. However, the impact of FN on immune regulation in the tumor microenvironment has not been fully elucidated.<h4>Methods</h4>The abundance of FN was measured in 99 stage III CRC tumor tissues using quantitative polymerase chain reaction. Gene expression profiles were assessed and annotated using consensus molecular subtypes (CMS), Gene Ontology (GO) analysis, and deconvolution of individual immune cell types in the context of FN abundance. Immune profiling for tumor infiltrating T cells isolated from human tumor tissues was analyzed using flow cytometry. <i>Ex vivo</i> tumor-infiltrating T cells were stimulated in2023-01-01T00:00:00Z20232025-04-19T07:01:58.065Z2025-04-19T07:01:58.065ZS-EPMC100280793696004210.3389/fcimb.2023.1101291