{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hsu YY"],"funding":["University of Michigan","NIBIB NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS","National Institute of Biomedical Imaging and Bioengineering"],"pagination":["12-18"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10028359"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Plasma membrane tension functions as a global physical organizer of cellular activities. Technical limitations of current membrane tension measurement techniques have hampered in-depth investigation of cellular membrane biophysics and the role of plasma membrane tension in regulating cellular processes. Here, we develop an optical membrane tension reporter by repurposing an <i>E. coli</i> mechanosensitive channel via insertion of circularly permuted GFP (cpGFP), which undergoes a large conformational rearrangement associated with channel activation and thus fluorescence intensity changes under increased membrane tension."],"journal":["ACS sensors"],"pubmed_title":["Mechanosensitive Channel-Based Optical Membrane Tension Reporter."],"pmcid":["PMC10028359"],"funding_grant_id":["R21 GM134157","R01 EB030031","R01EB030031","R21GM 134167","R21 GM103467"],"pubmed_authors":["Hsu YY","Fu J","Liu AP","Resto Irizarry AM"],"additional_accession":[]},"is_claimable":false,"name":"Mechanosensitive Channel-Based Optical Membrane Tension Reporter.","description":"Plasma membrane tension functions as a global physical organizer of cellular activities. Technical limitations of current membrane tension measurement techniques have hampered in-depth investigation of cellular membrane biophysics and the role of plasma membrane tension in regulating cellular processes. Here, we develop an optical membrane tension reporter by repurposing an <i>E. coli</i> mechanosensitive channel via insertion of circularly permuted GFP (cpGFP), which undergoes a large conformational rearrangement associated with channel activation and thus fluorescence intensity changes under increased membrane tension.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-18T15:29:30.764Z","creation":"2025-04-07T02:10:33.094Z"},"accession":"S-EPMC10028359","cross_references":{"pubmed":["36608338"],"doi":["10.1021/acssensors.2c01921"]}}