{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["18(3)"],"submitter":["Koehler C"],"funding":["European Research Council","VERAXA Biotech GmbH"],"pubmed_abstract":["Due to their target specificity, antibody-drug conjugates─monoclonal antibodies conjugated to a cytotoxic moiety─are efficient therapeutics that can kill malignant cells overexpressing a target gene. Linking an antibody with radioisotopes (radioimmunoconjugates) enables powerful diagnostics and/or closely related therapeutic applications, depending on the isotope. To generate site-specific radioimmunoconjugates, we utilized genetic code expansion and subsequent conjugation by inverse electron-demand Diels-Alder cycloaddition reactions. We show that, using this approach, site-specific labeling of trastuzumab with either zirconium-89 (<sup>89</sup>Zr) for diagnostics or lutetium-177 (<sup>177</sup>Lu) for therapeutics yields efficient radioimmunoconjugates. Positron emission tomography imagi"],"journal":["ACS chemical biology"],"pagination":["443-448"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10029752"],"repository":["biostudies-literature"],"pubmed_title":["Genetic Code Expansion for Site-Specific Labeling of Antibodies with Radioisotopes."],"pmcid":["PMC10029752"],"pubmed_authors":["Pektor S","Waldmann C","Bausbacher N","Koehler C","Sauter PF","Klasen B","Lemke EA","Miederer M"],"additional_accession":[]},"is_claimable":false,"name":"Genetic Code Expansion for Site-Specific Labeling of Antibodies with Radioisotopes.","description":"Due to their target specificity, antibody-drug conjugates─monoclonal antibodies conjugated to a cytotoxic moiety─are efficient therapeutics that can kill malignant cells overexpressing a target gene. Linking an antibody with radioisotopes (radioimmunoconjugates) enables powerful diagnostics and/or closely related therapeutic applications, depending on the isotope. To generate site-specific radioimmunoconjugates, we utilized genetic code expansion and subsequent conjugation by inverse electron-demand Diels-Alder cycloaddition reactions. We show that, using this approach, site-specific labeling of trastuzumab with either zirconium-89 (<sup>89</sup>Zr) for diagnostics or lutetium-177 (<sup>177</sup>Lu) for therapeutics yields efficient radioimmunoconjugates. Positron emission tomography imagi","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-18T15:29:04.915Z","creation":"2025-04-07T02:12:35.555Z"},"accession":"S-EPMC10029752","cross_references":{"pubmed":["36889678"],"doi":["10.1021/acschembio.2c00634"]}}