{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Robertson SN"],"funding":["Ministerio de Ciencia, Innovaci?n y Universidades","National Biofilms Innovation Centre","University of Nottingham","Biotechnology and Biological Sciences Research Council"],"pagination":["5079-5086"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10034741"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["95(11)"],"pubmed_abstract":["Previously, metabolites diffused or secreted from microbial samples have been analyzed via liquid chromatography-mass spectrometry (LC-MS) approaches following lengthy extraction protocols. Here, we present a model system for growing biofilms on discs before utilizing rapid and direct surface sampling MS, namely, liquid extraction surface analysis, to study the microbial exometabolome. One of the benefits of this approach is its surface-specific nature, enabling mimicking biofilm formation in a way that the study of planktonic liquid cultures cannot imitate. Even though <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), <i>Staphylococcus aureus (S. aureus),</i> and <i>Candida albicans</i> (<i>C. albicans</i>) have been studied previously in isolation, very few studies consider the compl"],"journal":["Analytical chemistry"],"pubmed_title":["Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry."],"pmcid":["PMC10034741"],"funding_grant_id":["BB/R012415/1","NBIC Interdisciplinary Research Fellow Robertson"],"pubmed_authors":["Griffiths RL","Soukarieh F","Romero M","Robertson SN","Camara M","White TM"],"additional_accession":[]},"is_claimable":false,"name":"Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry.","description":"Previously, metabolites diffused or secreted from microbial samples have been analyzed via liquid chromatography-mass spectrometry (LC-MS) approaches following lengthy extraction protocols. Here, we present a model system for growing biofilms on discs before utilizing rapid and direct surface sampling MS, namely, liquid extraction surface analysis, to study the microbial exometabolome. One of the benefits of this approach is its surface-specific nature, enabling mimicking biofilm formation in a way that the study of planktonic liquid cultures cannot imitate. Even though <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>), <i>Staphylococcus aureus (S. aureus),</i> and <i>Candida albicans</i> (<i>C. albicans</i>) have been studied previously in isolation, very few studies consider the compl","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-27T03:05:40.782Z","creation":"2025-04-06T18:45:21.437Z"},"accession":"S-EPMC10034741","cross_references":{"pubmed":["36881460"],"doi":["10.1021/acs.analchem.2c05703"]}}