{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["van Tienhoven R"],"funding":["The Wanek Family Project for Type 1 Diabetes","Leona M. and Harry B. Helmsley Charitable Trust","Stichting Diabetes Onderzoek Nederland","IMI2-JU","NIDDK NIH HHS","Juvenile Diabetes Research Foundation","ZonMw","Diabetes Fonds"],"pagination":["884-896"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10036285"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["66(5)"],"pubmed_abstract":["<h4>Aims/hypothesis</h4>Transcriptome analyses revealed insulin-gene-derived transcripts in non-beta endocrine islet cells. We studied alternative splicing of human INS mRNA in pancreatic islets.<h4>Methods</h4>Alternative splicing of insulin pre-mRNA was determined by PCR analysis performed on human islet RNA and single-cell RNA-seq analysis. Antisera were generated to detect insulin variants in human pancreatic tissue using immunohistochemistry, electron microscopy and single-cell western blot to confirm the expression of insulin variants. Cytotoxic T lymphocyte (CTL) activation was determined by MIP-1β release.<h4>Results</h4>We identified an alternatively spliced INS product. This variant encodes the complete insulin signal peptide and B chain and an alternative C-terminus that largely"],"journal":["Diabetologia"],"pubmed_title":["Presence of immunogenic alternatively spliced insulin gene product in human pancreatic delta cells."],"pmcid":["PMC10036285"],"funding_grant_id":["U24 DK098085","G-2108-04793","5-SRA-2018-557-Q-R","91111.006"],"pubmed_authors":["Kracht MJL","Thomaidou S","Carlotti F","Riojas JPR","de Koning EJP","Roep BO","van der Slik AR","Giepmans BNG","Nelson MS","Zaldumbide A","van Tienhoven R","Hoeben RC","Wolters AHG"],"additional_accession":[]},"is_claimable":false,"name":"Presence of immunogenic alternatively spliced insulin gene product in human pancreatic delta cells.","description":"<h4>Aims/hypothesis</h4>Transcriptome analyses revealed insulin-gene-derived transcripts in non-beta endocrine islet cells. We studied alternative splicing of human INS mRNA in pancreatic islets.<h4>Methods</h4>Alternative splicing of insulin pre-mRNA was determined by PCR analysis performed on human islet RNA and single-cell RNA-seq analysis. Antisera were generated to detect insulin variants in human pancreatic tissue using immunohistochemistry, electron microscopy and single-cell western blot to confirm the expression of insulin variants. Cytotoxic T lymphocyte (CTL) activation was determined by MIP-1β release.<h4>Results</h4>We identified an alternatively spliced INS product. This variant encodes the complete insulin signal peptide and B chain and an alternative C-terminus that largely","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-07-14T19:09:03.973Z","creation":"2025-02-19T00:49:41.469Z"},"accession":"S-EPMC10036285","cross_references":{"pubmed":["36884057"],"doi":["10.1007/s00125-023-05882-y"]}}