{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(1)"],"submitter":["Komatsu J"],"funding":["Scipio bioscience"],"pubmed_abstract":["Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology optimized for samples of fresh cells. Complexes of one cell paired with one barcoded bead are stabilized by a chemical linker and dispersed in a hydrogel in the liquid state. Upon gelation on ice the complexes are immobilized and physically separated without requiring nanowells or droplets. Cell lysis is triggered by detergent diffusion, and RNA molecules are captured on the adjacent barcoded beads for further processing with reverse transcription and preparation for cDNA sequencing. As a proof of concept, analysis of PBMC using RevGel-seq achieves results similar to microfluidic-based technologies when using the same original sample and the same data analysis software. In additio"],"journal":["Scientific reports"],"pagination":["4866"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10039079"],"repository":["biostudies-literature"],"pubmed_title":["RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding."],"pmcid":["PMC10039079"],"pubmed_authors":["Ismail J","Chaumat P","Saffarian A","Bussell X","Dimitrova D","Walrafen P","Morf J","Eckert H","Cico A","Graindorge A","Guinin M","Blondeau B","Poncin R","Fernandez N","Moretto V","Edelstein S","Cathaly L","Ulveling D","Lipin S","Majello S","Baldivia E","Andre B","Liboz A","Roblot N","Guillemain G","Fourne Y","Godet U","Caille O","Vayaboury A","Guerin F","Komatsu J","Le Bohec M","Beaupere C"],"additional_accession":[]},"is_claimable":false,"name":"RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding.","description":"Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology optimized for samples of fresh cells. Complexes of one cell paired with one barcoded bead are stabilized by a chemical linker and dispersed in a hydrogel in the liquid state. Upon gelation on ice the complexes are immobilized and physically separated without requiring nanowells or droplets. Cell lysis is triggered by detergent diffusion, and RNA molecules are captured on the adjacent barcoded beads for further processing with reverse transcription and preparation for cDNA sequencing. As a proof of concept, analysis of PBMC using RevGel-seq achieves results similar to microfluidic-based technologies when using the same original sample and the same data analysis software. In additio","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-26T13:05:14.698Z","creation":"2025-04-06T14:10:29.047Z"},"accession":"S-EPMC10039079","cross_references":{"pubmed":["36964177"],"doi":["10.1038/s41598-023-31915-y"]}}