biostudies-literaturePaz HAUniversity of Colorado Anschutz Medical CampusNIDDK NIH HHSAnschutz Health and Wellness CenterUnited States Department of Agriculture-Agricultural Research ServiceNIH HHSe15646https://www.ebi.ac.uk/biostudies/studies/S-EPMC10040403biostudies-literatureUnknown11(6)Activation of thermogenic adipose tissue depots has been linked to improved metabolism and weight loss. To study the molecular regulation of adipocyte thermogenesis, we performed RNA-Seq on brown adipose tissue (BAT), gonadal white adipose tissue (gWAT), and inguinal white adipose tissue (iWAT) from mice treated with β3-adrenoreceptor agonist CL316,243 (CL). Our analysis revealed diverse transcriptional profile and identified pathways in response to CL treatment. Differentially expressed genes (DEGs) in iWATCL were associated with the upregulation of pathways involved in cellular immune responses and with the upregulation of the browning program. We identified 39 DEGs in beige adipose which included certain heat shock proteins (Hspa1a and Hspa1b), and others suggesting potential associatioPhysiological reportsBeta-adrenergic agonist induces unique transcriptomic signature in inguinal white adipose tissue.PMC10040403P20GM1090965 P30DK048520-271 R01HD102726-01A16026-51000-010-05SUL1 TR003107R01-DK-084225Wankhade UDPaz HALoy HDShankar KPilkington ACZhong YfalseBeta-adrenergic agonist induces unique transcriptomic signature in inguinal white adipose tissue.Activation of thermogenic adipose tissue depots has been linked to improved metabolism and weight loss. To study the molecular regulation of adipocyte thermogenesis, we performed RNA-Seq on brown adipose tissue (BAT), gonadal white adipose tissue (gWAT), and inguinal white adipose tissue (iWAT) from mice treated with β3-adrenoreceptor agonist CL316,243 (CL). Our analysis revealed diverse transcriptional profile and identified pathways in response to CL treatment. Differentially expressed genes (DEGs) in iWATCL were associated with the upregulation of pathways involved in cellular immune responses and with the upregulation of the browning program. We identified 39 DEGs in beige adipose which included certain heat shock proteins (Hspa1a and Hspa1b), and others suggesting potential associatio2023-01-01T00:00:00Z2023 Mar2025-04-27T03:06:06.471Z2025-04-06T18:46:23.091ZS-EPMC100404033696723710.14814/phy2.15646