{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Sukegawa S"],"pubmed_abstract":["Cas9 derived from <i>Streptococcus pyogenes</i> (SpCas9) is used widely in genome editing using the CRISPR-Cas system due to its high activity, but is a relatively large molecule (1,368 amino acid (a.a.) residues). Recently, targeted mutagenesis in human cells and maize using Cas12f derived from <i>Syntrophomonas palmitatica</i> (SpCas12f)-a very small Cas of 497 a.a, which is a more suitable size for virus vectors-was reported. However, there are no reports of genome editing using SpCas12f in crops other than maize. In this study, we applied SpCas12f to genome editing in rice-one of the most important staple crops in the world. An expression vector encoding rice codon-optimized SpCas12f and sgRNA for <i>OsTubulin</i> as a target was introduced into rice calli by <i>Agrobacterium</i>-media"],"journal":["Frontiers in genome editing"],"pagination":["1138843"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10040665"],"repository":["biostudies-literature"],"pubmed_title":["Genome editing in rice mediated by miniature size Cas nuclease SpCas12f."],"pmcid":["PMC10040665"],"pubmed_authors":["Sukegawa S","Nureki O","Toki S","Saika H"],"additional_accession":[]},"is_claimable":false,"name":"Genome editing in rice mediated by miniature size Cas nuclease SpCas12f.","description":"Cas9 derived from <i>Streptococcus pyogenes</i> (SpCas9) is used widely in genome editing using the CRISPR-Cas system due to its high activity, but is a relatively large molecule (1,368 amino acid (a.a.) residues). Recently, targeted mutagenesis in human cells and maize using Cas12f derived from <i>Syntrophomonas palmitatica</i> (SpCas12f)-a very small Cas of 497 a.a, which is a more suitable size for virus vectors-was reported. However, there are no reports of genome editing using SpCas12f in crops other than maize. In this study, we applied SpCas12f to genome editing in rice-one of the most important staple crops in the world. An expression vector encoding rice codon-optimized SpCas12f and sgRNA for <i>OsTubulin</i> as a target was introduced into rice calli by <i>Agrobacterium</i>-media","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-19T01:46:00.672Z","creation":"2025-02-19T03:01:28.026Z"},"accession":"S-EPMC10040665","cross_references":{"pubmed":["36992681"],"doi":["10.3389/fgeed.2023.1138843"]}}