biostudies-literatureZhou YNatural Science Foundation of Shannxi Province, ChinaFundamental Research Funds for the Central UniversitiesKey R&D Plan of Shaanxi Provincee202200259https://www.ebi.ac.uk/biostudies/studies/S-EPMC10041546biostudies-literatureUnknown12(3)Protein analysis is an important approach for disease diagnosis, in which sample pretreatment is an essential step since protein samples are often complex and many protein biomarkers are of low abundance. Here, given the good openness and light transmission of liquid plasticine (LP), which is a liquid entity formed by SiO₂ nanoparticles and encapsulated aqueous solution, we developed a LP-based field-amplified sample stacking (FASS) system for protein enrichment. The system was composed of a LP container, a sample solution and a Tris-HCl solution containing hydroxyethyl cellulose (HEC). The system design, mechanism investigation, optimization of experimental parameters and characterization of LP-FASS performance for protein enrichment were well studied. Under the optimized experChemistryOpenLiquid Plasticine-Based Electrokinetic Enrichment of Proteins.PMC10041546SY6J0072020JC-0622127803HZ2021SF-168Li FNiu JZhou YfalseLiquid Plasticine-Based Electrokinetic Enrichment of Proteins.Protein analysis is an important approach for disease diagnosis, in which sample pretreatment is an essential step since protein samples are often complex and many protein biomarkers are of low abundance. Here, given the good openness and light transmission of liquid plasticine (LP), which is a liquid entity formed by SiO₂ nanoparticles and encapsulated aqueous solution, we developed a LP-based field-amplified sample stacking (FASS) system for protein enrichment. The system was composed of a LP container, a sample solution and a Tris-HCl solution containing hydroxyethyl cellulose (HEC). The system design, mechanism investigation, optimization of experimental parameters and characterization of LP-FASS performance for protein enrichment were well studied. Under the optimized exper2023-01-01T00:00:00Z2023 Mar2025-04-18T13:32:29.537Z2025-04-06T23:16:29.679ZS-EPMC100415463697110510.1002/open.202200259