biostudies-literatureIurescia Mthe European Union's Horizon 2020 Re-search and Innovation programmeEuropean UnionEMIDA ERA-NET (FP7-KBBE) first transnational research callMethicillin-resistant Staphylococcus aureus lineages in pri-mary productions: multi-host pathogen, spill-over and spill-back between animals and hu-mans?European Union’s Horizon 2020 Research and Innovation programme530https://www.ebi.ac.uk/biostudies/studies/S-EPMC10044604biostudies-literatureUnknown12(3)The <i>cfr</i> genes encode for a 23S rRNA methyltransferase, conferring a multiresistance phenotype to phenicol, lincosamide, oxazolidinone, pleuromutilin, and streptogramin A antibiotics. These genes have been described in staphylococci, including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). In this study, we retrospectively performed an in-depth genomic characterisation of three <i>cfr</i>-positive, multidrug-resistant (MDR) livestock-associated (LA) MRSA clonal complexes (CCs) 1 and 398 detected in different Italian pig holdings (2008-2011) during population studies on Italian livestock (2008-2014). We used a combined Illumina and Oxford Nanopore Technologies (ONT) whole genome sequencing (WGS) approach on two isolates (the 2008 CC1 and the 2010 CC398 isolates, but not thAntibiotics (Basel, Switzerland)Genomics Insight into <i>cfr</i>-Mediated Linezolid-Resistant LA-MRSA in Italian Pig Holdings.PMC1004460468No 773830: One Health European Joint Programme.EMIDA ERA-NET (FP7-KBBE) first transnational research call, Project Title: "Methicillin-resistant Staphylococcus aureus lineages in primary productions: multi-host pathogen, spill-over and spill-back between animals and humans?", "LA-MRSA" (acronym title)773830"LA-MRSA" (acronym title) ID 68.Iurescia MAlba PBuccella CCarfora VDiaconu ELCaprioli AGiacomi AFranco AFeltrin FOnorati RBattisti AfalseGenomics Insight into <i>cfr</i>-Mediated Linezolid-Resistant LA-MRSA in Italian Pig Holdings.The <i>cfr</i> genes encode for a 23S rRNA methyltransferase, conferring a multiresistance phenotype to phenicol, lincosamide, oxazolidinone, pleuromutilin, and streptogramin A antibiotics. These genes have been described in staphylococci, including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). In this study, we retrospectively performed an in-depth genomic characterisation of three <i>cfr</i>-positive, multidrug-resistant (MDR) livestock-associated (LA) MRSA clonal complexes (CCs) 1 and 398 detected in different Italian pig holdings (2008-2011) during population studies on Italian livestock (2008-2014). We used a combined Illumina and Oxford Nanopore Technologies (ONT) whole genome sequencing (WGS) approach on two isolates (the 2008 CC1 and the 2010 CC398 isolates, but not th2023-01-01T00:00:00Z2023 Mar2026-04-08T13:57:43.871Z2024-11-12T20:47:32.086ZS-EPMC100446043697839710.3390/antibiotics12030530