biostudies-literatureWang ZNational Institute of Environmental Health SciencesNIEHS NIH HHSNatural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of China1133-1146https://www.ebi.ac.uk/biostudies/studies/S-EPMC10045874biostudies-literatureUnknown97(4)Intestinal organoid may serve as an alternative model for toxicity testing. However, the linkage between specific morphological alterations in organoids and chemical-induced toxicity has yet to be defined. Here, we generated C57BL/6 mouse intestinal organoids and conducted a morphology-based analysis on chemical-induced toxicity. Alterations in morphology were characterized by large spheroids, hyperplastic organoids, small spheroids, and protrusion-loss organoids, which responded in a concentration-dependent manner to the treatment of four metal(loid)s including cadmium (Cd), lead (Pb), hexavalent chromium (Cr-VI), and inorganic trivalent arsenic (iAs-III). Notably, alterations in organoid morphology characterized by abnormal morphology rate were correlated with specific intestinal toxic eArchives of toxicologyMorphological alterations in C57BL/6 mouse intestinal organoids as a tool for predicting chemical-induced toxicity.PMC100458742021A151501035082204080R01 ES10563R01 ES00733182073580R01 ES07331Guo ZLi DXing XChen LPang YZhang RZhang QLi MJiang YYe LAschner MJiang XChen WChen SWang ZfalseMorphological alterations in C57BL/6 mouse intestinal organoids as a tool for predicting chemical-induced toxicity.Intestinal organoid may serve as an alternative model for toxicity testing. However, the linkage between specific morphological alterations in organoids and chemical-induced toxicity has yet to be defined. Here, we generated C57BL/6 mouse intestinal organoids and conducted a morphology-based analysis on chemical-induced toxicity. Alterations in morphology were characterized by large spheroids, hyperplastic organoids, small spheroids, and protrusion-loss organoids, which responded in a concentration-dependent manner to the treatment of four metal(loid)s including cadmium (Cd), lead (Pb), hexavalent chromium (Cr-VI), and inorganic trivalent arsenic (iAs-III). Notably, alterations in organoid morphology characterized by abnormal morphology rate were correlated with specific intestinal toxic e2023-01-01T00:00:00Z2023 Apr2025-04-26T19:39:57.128Z2025-04-06T16:12:14.877ZS-EPMC100458743680689510.1007/s00204-023-03451-1