biostudies-literatureTran Nguyen Minh HMinistry of Science and TechnologyMinistry of Science and Technology, TaiwanAcademia SinicaCouncil of AgricultureCouncil of Agriculture , Taiwan346https://www.ebi.ac.uk/biostudies/studies/S-EPMC10045918biostudies-literatureUnknown10(3)Gut microbiota play a key role in health maintenance and disease pathogenesis in animals. Dietary phytochemicals are crucial factors shaping gut bacteria. Here, we investigated the function and mechanism of a phytogenic formulation, EUBIO-BPSG (BP), in laying hens. We found that BP dose-dependently improved health and egg production in 54-week-old hens. Furthermore, BP was correlated with increased fecal <i>Lactobacillus</i>, decreased <i>Escherichia coli</i> and <i>Salmonella enterica</i>, and reduced antibiotic resistance (AR) and antibiotic resistance genes (ARG) in chicken stools. The 16S rDNA data showed that BP increased seven genera of probiotics and reduced 13 genera of pathogens in chicken feces. In vitro co-culture experiments showed that BP at 4 µg/mL and above promoted growth oBioengineering (Basel, Switzerland)A Novel Phytogenic Formulation, EUBIO-BPSG, as a Promising One Health Approach to Replace Antibiotics and Promote Reproduction Performance in Laying Hens.PMC10045918MOST 110-2321-B-001-015112AS-1.6.1-ST-a5MOST 109-2311-B-001-040111AS-1.6.1-ST-aIAS-KPQ-110-ITAR-09Yang GOu BRChang THYang WCLiang YCPeng TCLin CYTran Nguyen Minh HKuo TFHo CHLin WYYang YLYang CWfalseA Novel Phytogenic Formulation, EUBIO-BPSG, as a Promising One Health Approach to Replace Antibiotics and Promote Reproduction Performance in Laying Hens.Gut microbiota play a key role in health maintenance and disease pathogenesis in animals. Dietary phytochemicals are crucial factors shaping gut bacteria. Here, we investigated the function and mechanism of a phytogenic formulation, EUBIO-BPSG (BP), in laying hens. We found that BP dose-dependently improved health and egg production in 54-week-old hens. Furthermore, BP was correlated with increased fecal <i>Lactobacillus</i>, decreased <i>Escherichia coli</i> and <i>Salmonella enterica</i>, and reduced antibiotic resistance (AR) and antibiotic resistance genes (ARG) in chicken stools. The 16S rDNA data showed that BP increased seven genera of probiotics and reduced 13 genera of pathogens in chicken feces. In vitro co-culture experiments showed that BP at 4 µg/mL and above promoted growth o2023-01-01T00:00:00Z2023 Mar2026-04-08T13:24:42.103Z2024-11-05T15:04:50.272ZS-EPMC100459183697873710.3390/bioengineering10030346