{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Erkes A"],"funding":["Agence Nationale de la Recherche","Deutsche Forschungsgemeinschaft","Thüringer Ministerium für Wirtschaft, Wissenschaft und Digitale Gesellschaft","Martin-Luther-Universität Halle-Wittenberg"],"pagination":["151"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10045945"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(1)"],"pubmed_abstract":["<h4>Background</h4>Most plant-pathogenic Xanthomonas bacteria harbor transcription activator-like effector (TALE) genes, which function as transcriptional activators of host plant genes and support infection. The entire repertoire of up to 29 TALE genes of a Xanthomonas strain is also referred to as TALome. The DNA-binding domain of TALEs is comprised of highly conserved repeats and TALE genes often occur in gene clusters, which precludes the assembly of TALE-carrying Xanthomonas genomes based on standard sequencing approaches.<h4>Results</h4>Here, we report the successful assembly of the 5 Mbp genomes of five Xanthomonas strains from Oxford Nanopore Technologies (ONT) sequencing data. For one of these strains, Xanthomonas oryzae pv. oryzae (Xoo) PXO35, we illustrate why Illumina short rea"],"journal":["BMC genomics"],"pubmed_title":["Assembling highly repetitive Xanthomonas TALomes using Oxford Nanopore sequencing."],"pmcid":["PMC10045945"],"funding_grant_id":["DigLeben-5575/10-9","ANR-2010-BLAN-1723","GR 4587/1-2","FZT 118/2","BO 1496/8-2"],"pubmed_authors":["Krautwurst S","Wilson GG","Erkes A","Zarkovic M","Morgan RD","Holzer M","Grau J","Boch J","Marz M","Grove RP","Koebnik R"],"additional_accession":[]},"is_claimable":false,"name":"Assembling highly repetitive Xanthomonas TALomes using Oxford Nanopore sequencing.","description":"<h4>Background</h4>Most plant-pathogenic Xanthomonas bacteria harbor transcription activator-like effector (TALE) genes, which function as transcriptional activators of host plant genes and support infection. The entire repertoire of up to 29 TALE genes of a Xanthomonas strain is also referred to as TALome. The DNA-binding domain of TALEs is comprised of highly conserved repeats and TALE genes often occur in gene clusters, which precludes the assembly of TALE-carrying Xanthomonas genomes based on standard sequencing approaches.<h4>Results</h4>Here, we report the successful assembly of the 5 Mbp genomes of five Xanthomonas strains from Oxford Nanopore Technologies (ONT) sequencing data. For one of these strains, Xanthomonas oryzae pv. oryzae (Xoo) PXO35, we illustrate why Illumina short rea","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-05-29T03:14:24.343Z","creation":"2025-02-19T02:38:25.101Z"},"accession":"S-EPMC10045945","cross_references":{"pubmed":["36973643"],"doi":["10.1186/s12864-023-09228-1"]}}