biostudies-literatureGutierrez-Galvez LEUMINECOSpanish Ministry of UniversitiesMINECO,SEVERO OCHOACMCommunity of MadridSpanish Ministry of Economy and Competitiveness348https://www.ebi.ac.uk/biostudies/studies/S-EPMC10046281biostudies-literatureUnknown13(3)In this work, we present the combination of two different types of nanomaterials, 2D molybdenum disulfide nanosheets (MoS₂-NS) and zero-dimensional carbon nanodots (CDs), for the development of a new electrochemiluminescence (ECL) platform for the early detection and quantification of the biomarker human epidermal growth factor receptor 2 (HER2), whose overexpression is associated with breast cancer. MoS₂-NS are used as an immobilization platform for the thiolated aptamer, which can recognize the HER2 epitope peptide with high affinity, and CDs act as coreactants of the anodic oxidation of the luminophore [Ru(bpy)₃]²⁺. The HER2 biomarker is detected by changes in the ECL signal of the [Ru(bpy)₃]²⁺/CD system, with a low detection limit of 1.84 fg/mL and a wide linear range. The proposed method has been successfully applied to detect the HER2 biomarker in human serum samples.BiosensorsMoS₂-Carbon Nanodots as a New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection.PMC10046281PID2020-116728RB-I00, PID2020-116661RB-I00, CTQ2015-71955-REDT (ELECTROBIONET)Grant CEX2020-001039-STRANSNANOAVANSENS, S2018/NMT-4349, and FotoArt (P2018/NMT-4367project S2018/NMT-4291 TEC2SPACEFPU19/06309project CSIC13-4E-1794FEDER, FSEPerez EMSulleiro MVGarcia-Mendiola TLorenzo EGutierrez-Galvez LGutierrez-Sanchez CLuna MGarcia-Nieto DfalseMoS₂-Carbon Nanodots as a New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection.In this work, we present the combination of two different types of nanomaterials, 2D molybdenum disulfide nanosheets (MoS₂-NS) and zero-dimensional carbon nanodots (CDs), for the development of a new electrochemiluminescence (ECL) platform for the early detection and quantification of the biomarker human epidermal growth factor receptor 2 (HER2), whose overexpression is associated with breast cancer. MoS₂-NS are used as an immobilization platform for the thiolated aptamer, which can recognize the HER2 epitope peptide with high affinity, and CDs act as coreactants of the anodic oxidation of the luminophore [Ru(bpy)₃]²⁺. The HER2 biomarker is detected by changes in the ECL signal of the [Ru(bpy)₃]²⁺/CD system, with a low detection limit of 1.84 fg/mL and a wide linear range. The proposed method has been successfully applied to detect the HER2 biomarker in human serum samples.2023-01-01T00:00:00Z2023 Mar2024-10-17T22:13:12.972Z2024-10-17T22:13:12.972ZS-EPMC100462813697956010.3390/bios13030348