{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lee I"],"funding":["Agricultural Research Service"],"pagination":["985"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10051117"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(6)"],"pubmed_abstract":["An electrochemical immunosensor has been developed for the rapid detection and identification of potentially harmful bacteria in food and environmental samples. This study aimed to fabricate a microwire-based electrochemical immunosensor (MEI sensor) for selective detection of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> in microbial cocktail samples using dielectrophoresis (DEP)-based cell concentration. A gold-coated tungsten microwire was functionalized by coating polyethylenimine, single-walled carbon nanotube (SWCNT) suspension, streptavidin, biotinylated antibodies, and then bovine serum albumin (BSA) solutions. Double-layered SWCNTs and 5% BSA solution were found to be optimized for enhanced signal enhancement and nonspecific binding barrier. The selective capture of <i>"],"journal":["Nanomaterials (Basel, Switzerland)"],"pubmed_title":["Selective Detection of <i>Escherichia coli</i> K12 and <i>Staphylococcus aureus</i> in Mixed Bacterial Communities Using a Single-Walled Carbon Nanotube (SWCNT)-Functionalized Electrochemical Immunosensor with Dielectrophoretic Concentration."],"pmcid":["PMC10051117"],"funding_grant_id":["58-2040-8-010"],"pubmed_authors":["Ho K","Auh JH","Wall MM","Li Y","So H","Lee I","Kim J","Jun S"],"additional_accession":[]},"is_claimable":false,"name":"Selective Detection of <i>Escherichia coli</i> K12 and <i>Staphylococcus aureus</i> in Mixed Bacterial Communities Using a Single-Walled Carbon Nanotube (SWCNT)-Functionalized Electrochemical Immunosensor with Dielectrophoretic Concentration.","description":"An electrochemical immunosensor has been developed for the rapid detection and identification of potentially harmful bacteria in food and environmental samples. This study aimed to fabricate a microwire-based electrochemical immunosensor (MEI sensor) for selective detection of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> in microbial cocktail samples using dielectrophoresis (DEP)-based cell concentration. A gold-coated tungsten microwire was functionalized by coating polyethylenimine, single-walled carbon nanotube (SWCNT) suspension, streptavidin, biotinylated antibodies, and then bovine serum albumin (BSA) solutions. Double-layered SWCNTs and 5% BSA solution were found to be optimized for enhanced signal enhancement and nonspecific binding barrier. The selective capture of <i>","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-19T00:01:34.368Z","creation":"2024-10-17T17:23:30.387Z"},"accession":"S-EPMC10051117","cross_references":{"pubmed":["36985878"],"doi":["10.3390/nano13060985"]}}