biostudies-literatureLee IAgricultural Research Service985https://www.ebi.ac.uk/biostudies/studies/S-EPMC10051117biostudies-literatureUnknown13(6)An electrochemical immunosensor has been developed for the rapid detection and identification of potentially harmful bacteria in food and environmental samples. This study aimed to fabricate a microwire-based electrochemical immunosensor (MEI sensor) for selective detection of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> in microbial cocktail samples using dielectrophoresis (DEP)-based cell concentration. A gold-coated tungsten microwire was functionalized by coating polyethylenimine, single-walled carbon nanotube (SWCNT) suspension, streptavidin, biotinylated antibodies, and then bovine serum albumin (BSA) solutions. Double-layered SWCNTs and 5% BSA solution were found to be optimized for enhanced signal enhancement and nonspecific binding barrier. The selective capture of <i>Nanomaterials (Basel, Switzerland)Selective Detection of <i>Escherichia coli</i> K12 and <i>Staphylococcus aureus</i> in Mixed Bacterial Communities Using a Single-Walled Carbon Nanotube (SWCNT)-Functionalized Electrochemical Immunosensor with Dielectrophoretic Concentration.PMC1005111758-2040-8-010Ho KAuh JHWall MMLi YSo HLee IKim JJun SfalseSelective Detection of <i>Escherichia coli</i> K12 and <i>Staphylococcus aureus</i> in Mixed Bacterial Communities Using a Single-Walled Carbon Nanotube (SWCNT)-Functionalized Electrochemical Immunosensor with Dielectrophoretic Concentration.An electrochemical immunosensor has been developed for the rapid detection and identification of potentially harmful bacteria in food and environmental samples. This study aimed to fabricate a microwire-based electrochemical immunosensor (MEI sensor) for selective detection of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> in microbial cocktail samples using dielectrophoresis (DEP)-based cell concentration. A gold-coated tungsten microwire was functionalized by coating polyethylenimine, single-walled carbon nanotube (SWCNT) suspension, streptavidin, biotinylated antibodies, and then bovine serum albumin (BSA) solutions. Double-layered SWCNTs and 5% BSA solution were found to be optimized for enhanced signal enhancement and nonspecific binding barrier. The selective capture of <i>2023-01-01T00:00:00Z2023 Mar2025-04-19T00:01:34.368Z2024-10-17T17:23:30.387ZS-EPMC100511173698587810.3390/nano13060985