biostudies-literatureBaur TEuropean Research Area Cofund on BioTechnologies685https://www.ebi.ac.uk/biostudies/studies/S-EPMC10053330biostudies-literatureUnknown11(3)Propionate is an important platform chemical that is available through petrochemical synthesis. Bacterial propionate formation is considered an alternative, as bacteria can convert waste substrates into valuable products. In this regard, research primarily focused on propionibacteria due to high propionate titers achieved from different substrates. Whether other bacteria could also be attractive producers is unclear, mostly because little is known about these strains. Therefore, two thus far less researched strains, <i>Anaerotignum propionicum</i> and <i>Anaerotignum neopropionicum</i>, were investigated with regard to their morphologic and metabolic features. Microscopic analyses revealed a negative Gram reaction despite a Gram-positive cell wall as well as surface layers for both strainsMicroorganismsNew Insights into the Physiology of the Propionate Producers <i>Anaerotignum propionicum</i> and <i>Anaerotignum neopropionicum</i> (Formerly <i>Clostridium propionicum</i> and <i>Clostridium neopropionicum</i>).PMC10053330161B0594ABaur TDurre PfalseNew Insights into the Physiology of the Propionate Producers <i>Anaerotignum propionicum</i> and <i>Anaerotignum neopropionicum</i> (Formerly <i>Clostridium propionicum</i> and <i>Clostridium neopropionicum</i>).Propionate is an important platform chemical that is available through petrochemical synthesis. Bacterial propionate formation is considered an alternative, as bacteria can convert waste substrates into valuable products. In this regard, research primarily focused on propionibacteria due to high propionate titers achieved from different substrates. Whether other bacteria could also be attractive producers is unclear, mostly because little is known about these strains. Therefore, two thus far less researched strains, <i>Anaerotignum propionicum</i> and <i>Anaerotignum neopropionicum</i>, were investigated with regard to their morphologic and metabolic features. Microscopic analyses revealed a negative Gram reaction despite a Gram-positive cell wall as well as surface layers for both strains2023-01-01T00:00:00Z2023 Mar2025-04-06T02:21:13.679Z2025-02-19T00:14:12.621ZS-EPMC100533303698525710.3390/microorganisms11030685