biostudies-literatureUnknownHolman CDNICHD NIH HHSNIDDK NIH HHSThe energy-burning capability of beige adipose tissue is a potential therapeutic tool for reducing obesity and metabolic disease, but this capacity is decreased by aging. Here, we evaluate the impact of aging on the profile and activity of adipocyte stem and progenitor cells (ASPCs) and adipocytes during the beiging process. We found that aging increases the expression of <i>Cd9</i> and other fibro-inflammatory genes in fibroblastic ASPCs and blocks their differentiation into beige adipocytes. Fibroblastic ASPC populations from young and aged mice were equally competent for beige differentiation <i>in vitro</i>, suggesting that environmental factors suppress adipogenesis <i>in vivo</i>. Examination of adipocytes by single nucleus RNA-sequencing identified compositional and transcriptional bioRxiv : the preprint server for biology2023.03.20.533514https://www.ebi.ac.uk/biostudies/studies/S-EPMC10055201biostudies-literatureAging impairs cold-induced beige adipogenesis and adipocyte metabolic reprogramming.PMC10055201R01 DK121801P30 DK046200P30 DK019525R01 DK120982P30 DK135043R01 DK123356RC2 DK116691P30 DK057521T32 DK007314T32 HD083185Holman CDSeale PRosen EDSakers APCheng LCalhoun RPJacobs CTsai LFein ECfalseAging impairs cold-induced beige adipogenesis and adipocyte metabolic reprogramming.The energy-burning capability of beige adipose tissue is a potential therapeutic tool for reducing obesity and metabolic disease, but this capacity is decreased by aging. Here, we evaluate the impact of aging on the profile and activity of adipocyte stem and progenitor cells (ASPCs) and adipocytes during the beiging process. We found that aging increases the expression of <i>Cd9</i> and other fibro-inflammatory genes in fibroblastic ASPCs and blocks their differentiation into beige adipocytes. Fibroblastic ASPC populations from young and aged mice were equally competent for beige differentiation <i>in vitro</i>, suggesting that environmental factors suppress adipogenesis <i>in vivo</i>. Examination of adipocytes by single nucleus RNA-sequencing identified compositional and transcriptional 2024-01-01T00:00:00Z2024 Feb2026-07-06T03:15:27.148Z2026-07-06T03:08:41.829ZS-EPMC100552013699333610.1101/2023.03.20.533514