biostudies-literatureUnknown13(3)Kashyap AWild species are weedy relatives and progenitors of cultivated crops, usually maintained in their centres of origin. They are rich sources of diversity as they possess many agriculturally important traits. In this study, we analysed 25 wild species and 5 U triangle species of <i>Brassica</i> for their potential tolerance against heat and drought stress during germination and in order to examine the early seedling stage. We identified the germplasms based on the mean membership function value (MFV), which was calculated from the tolerance index of shoot length, root length, and biochemical analysis. The study revealed that <i>B. napus</i> (GSC-6) could withstand high temperatures and drought. Other genotypes that were tolerant to the impact of heat stress were <i>B. tournefortii</i> (RBT 20Life (Basel, Switzerland)738https://www.ebi.ac.uk/biostudies/studies/S-EPMC10055847biostudies-literatureIndexing Resilience to Heat and Drought Stress in the Wild Relatives of Rapeseed-Mustard.PMC10055847Kashyap AKushwaha RBhattacharya RRana JCVishwakarma HGarg PTripathi SSharma JKumar RRKumari SGupta NCYadav RRao MfalseIndexing Resilience to Heat and Drought Stress in the Wild Relatives of Rapeseed-Mustard.Wild species are weedy relatives and progenitors of cultivated crops, usually maintained in their centres of origin. They are rich sources of diversity as they possess many agriculturally important traits. In this study, we analysed 25 wild species and 5 U triangle species of <i>Brassica</i> for their potential tolerance against heat and drought stress during germination and in order to examine the early seedling stage. We identified the germplasms based on the mean membership function value (MFV), which was calculated from the tolerance index of shoot length, root length, and biochemical analysis. The study revealed that <i>B. napus</i> (GSC-6) could withstand high temperatures and drought. Other genotypes that were tolerant to the impact of heat stress were <i>B. tournefortii</i> (RBT 202023-01-01T00:00:00Z2023 Mar2025-05-29T20:04:25.845Z2025-05-29T20:04:25.845ZS-EPMC100558473698389310.3390/life13030738