{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lei M"],"funding":["National Institutes of Health","NIGMS NIH HHS"],"pagination":["399-408"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10072783"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["120(2)"],"pubmed_abstract":["Synthetic cell-cell interaction systems can be useful for understanding multicellular communities or for screening binding molecules. We adapt a previously characterized set of synthetic cognate nanobody-antigen pairs to a yeast-bacteria coincubation format and use flow cytometry to evaluate cell-cell interactions mediated by binding between surface-displayed molecules. We further use fluorescence-activated cell sorting to enrich a specific yeast-displayed nanobody within a mixed yeast-display population. Finally, we demonstrate that this system supports the characterization of a therapeutically relevant nanobody-antigen interaction: a previously discovered nanobody that binds to the intimin protein expressed on the surface of enterohemorrhagic Escherichia coli. Overall, our findings indicate that the yeast-bacteria format supports efficient evaluation of ligand-target interactions. With further development, this format may facilitate systematic characterization and high-throughput discovery of bacterial surface-binding molecules."],"journal":["Biotechnology and bioengineering"],"pubmed_title":["Flow cytometric evaluation of yeast-bacterial cell-cell interactions."],"pmcid":["PMC10072783"],"funding_grant_id":["R35 GM133471"],"pubmed_authors":["Lei M","Nair NU","Trivedi VD","Lee K","Van Deventer JA"],"additional_accession":[]},"is_claimable":false,"name":"Flow cytometric evaluation of yeast-bacterial cell-cell interactions.","description":"Synthetic cell-cell interaction systems can be useful for understanding multicellular communities or for screening binding molecules. We adapt a previously characterized set of synthetic cognate nanobody-antigen pairs to a yeast-bacteria coincubation format and use flow cytometry to evaluate cell-cell interactions mediated by binding between surface-displayed molecules. We further use fluorescence-activated cell sorting to enrich a specific yeast-displayed nanobody within a mixed yeast-display population. Finally, we demonstrate that this system supports the characterization of a therapeutically relevant nanobody-antigen interaction: a previously discovered nanobody that binds to the intimin protein expressed on the surface of enterohemorrhagic Escherichia coli. Overall, our findings indicate that the yeast-bacteria format supports efficient evaluation of ligand-target interactions. With further development, this format may facilitate systematic characterization and high-throughput discovery of bacterial surface-binding molecules.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-07-12T03:04:25.38Z","creation":"2025-04-06T13:24:00.114Z"},"accession":"S-EPMC10072783","cross_references":{"pubmed":["36259110"],"doi":["10.1002/bit.28253"]}}