biostudies-literatureThye KLMinistry of Higher Education, Malaysia377-392https://www.ebi.ac.uk/biostudies/studies/S-EPMC10073391biostudies-literatureUnknown29(3)Utilisation of calcium lignosulfonate (CaLS) in <i>Vanilla planifolia</i> has been reported to improve shoot multiplication. However, mechanisms responsible for such observation remain unknown. Here, we elucidated the underlying mechanisms of CaLS in promoting shoot multiplication of <i>V. planifolia</i> via comparative proteomics, biochemical assays, and nutrient analysis. The proteome profile of CaLS-treated plants showed enhancement of several important cellular metabolisms such as photosynthesis, protein synthesis, Krebs cycle, glycolysis, gluconeogenesis, and carbohydrate synthesis. Further biochemical analysis recorded that CaLS increased Rubisco activity, hexokinase activity, isocitrate dehydrogenase activity, total carbohydrate content, glutamate synthase activity and total proteinPhysiology and molecular biology of plants : an international journal of functional plant biologyCalcium lignosulfonate modulates physiological and biochemical responses to enhance shoot multiplication in <i>Vanilla planifolia</i> Andrews.PMC10073391FRGS/1/2019/STG05/UPM/02/27Lai KSWan Abdullah WMANOng-Abdullah JLamasudin DUCheng WHLoh JYThye KLWee CYMohd Yusoff MHYfalseCalcium lignosulfonate modulates physiological and biochemical responses to enhance shoot multiplication in <i>Vanilla planifolia</i> Andrews.Utilisation of calcium lignosulfonate (CaLS) in <i>Vanilla planifolia</i> has been reported to improve shoot multiplication. However, mechanisms responsible for such observation remain unknown. Here, we elucidated the underlying mechanisms of CaLS in promoting shoot multiplication of <i>V. planifolia</i> via comparative proteomics, biochemical assays, and nutrient analysis. The proteome profile of CaLS-treated plants showed enhancement of several important cellular metabolisms such as photosynthesis, protein synthesis, Krebs cycle, glycolysis, gluconeogenesis, and carbohydrate synthesis. Further biochemical analysis recorded that CaLS increased Rubisco activity, hexokinase activity, isocitrate dehydrogenase activity, total carbohydrate content, glutamate synthase activity and total protein2023-01-01T00:00:00Z2023 Mar2026-07-15T02:41:27.386Z2025-04-04T20:40:31.595ZS-EPMC100733913703376410.1007/s12298-023-01293-w