{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(3)"],"submitter":["Treacy PJ"],"pubmed_abstract":["<h4>Background and objective</h4>Multi-photon microscopy (MPM) is a 3-dimension fluorescence imaging technique that combines the excitation of two low-energy photons, enabling less photo-bleaching and deeper penetration of the imaged tissue. Two signals are detected, autofluorescence (AF), from natural intracellular fluorophores [such as nicotinamide adenine dinucleotide phosphate (NADP) and flavine adenine dinucleotide (FAD) transformation], and second harmonic generation (SHG), a physical property of the laser enhancing non-centrosymmetric structures such as collagen fibers. MPM can give both visual and quantitative information of a fresh tissue (without the need of processing, cutting or staining the tissue), aiding in the progress towards optimizing a real-time imaging device. The obje"],"journal":["Translational andrology and urology"],"pagination":["508-518"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10080359"],"repository":["biostudies-literature"],"pubmed_title":["Value of multiphoton microscopy in uro-oncology: a narrative review."],"pmcid":["PMC10080359"],"pubmed_authors":["Tewari AK","Khosla A","Tsavaras N","Durand M","Falagario UG","Treacy PJ","Kyprianou N","Wiklund P"],"additional_accession":[]},"is_claimable":false,"name":"Value of multiphoton microscopy in uro-oncology: a narrative review.","description":"<h4>Background and objective</h4>Multi-photon microscopy (MPM) is a 3-dimension fluorescence imaging technique that combines the excitation of two low-energy photons, enabling less photo-bleaching and deeper penetration of the imaged tissue. Two signals are detected, autofluorescence (AF), from natural intracellular fluorophores [such as nicotinamide adenine dinucleotide phosphate (NADP) and flavine adenine dinucleotide (FAD) transformation], and second harmonic generation (SHG), a physical property of the laser enhancing non-centrosymmetric structures such as collagen fibers. MPM can give both visual and quantitative information of a fresh tissue (without the need of processing, cutting or staining the tissue), aiding in the progress towards optimizing a real-time imaging device. The obje","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-19T14:30:21.631Z","creation":"2025-04-19T14:30:21.631Z"},"accession":"S-EPMC10080359","cross_references":{"pubmed":["37032746"],"doi":["10.21037/tau-21-973"]}}