biostudies-literatureUnknown12(3)Treacy PJ<h4>Background and objective</h4>Multi-photon microscopy (MPM) is a 3-dimension fluorescence imaging technique that combines the excitation of two low-energy photons, enabling less photo-bleaching and deeper penetration of the imaged tissue. Two signals are detected, autofluorescence (AF), from natural intracellular fluorophores [such as nicotinamide adenine dinucleotide phosphate (NADP) and flavine adenine dinucleotide (FAD) transformation], and second harmonic generation (SHG), a physical property of the laser enhancing non-centrosymmetric structures such as collagen fibers. MPM can give both visual and quantitative information of a fresh tissue (without the need of processing, cutting or staining the tissue), aiding in the progress towards optimizing a real-time imaging device. The objeTranslational andrology and urology508-518https://www.ebi.ac.uk/biostudies/studies/S-EPMC10080359biostudies-literatureValue of multiphoton microscopy in uro-oncology: a narrative review.PMC10080359Tewari AKKhosla ATsavaras NDurand MFalagario UGTreacy PJKyprianou NWiklund PfalseValue of multiphoton microscopy in uro-oncology: a narrative review.<h4>Background and objective</h4>Multi-photon microscopy (MPM) is a 3-dimension fluorescence imaging technique that combines the excitation of two low-energy photons, enabling less photo-bleaching and deeper penetration of the imaged tissue. Two signals are detected, autofluorescence (AF), from natural intracellular fluorophores [such as nicotinamide adenine dinucleotide phosphate (NADP) and flavine adenine dinucleotide (FAD) transformation], and second harmonic generation (SHG), a physical property of the laser enhancing non-centrosymmetric structures such as collagen fibers. MPM can give both visual and quantitative information of a fresh tissue (without the need of processing, cutting or staining the tissue), aiding in the progress towards optimizing a real-time imaging device. The obje2023-01-01T00:00:00Z2023 Mar2025-04-19T14:30:21.631Z2025-04-19T14:30:21.631ZS-EPMC100803593703274610.21037/tau-21-973