biostudies-literatureLi JNational Natural Sciences Foundation of China103https://www.ebi.ac.uk/biostudies/studies/S-EPMC10082527biostudies-literatureUnknown24(1)<h4>Background</h4>Epithelial barrier is important for asthma development by shaping immune responses. Airway expressing-IL-1 receptor-associated kinase (IRAK)-M of Toll-like receptor pathway was involved in immunoregulation of airway inflammation through influencing activities of macrophages and dendritic cells or T cell differentiation. Whether IRAK-M has effect on cellular immunity in airway epithelial cells upon stimulation remains unclear.<h4>Methods</h4>We modeled cellular inflammation induced by IL-1β, TNF-α, IL-33, and house dust mite (HDM) in BEAS-2B and A549 cells. Cytokine production and pathway activation were used to reflect the effects of IRAK-M siRNA knockdown on epithelial immunity. Genotyping an asthma-susceptible IRAK-M SNP rs1624395 and measurement of serum CXCL10 levelsRespiratory researchIRAK-M has effects in regulation of lung epithelial inflammation.PMC1008252781170040304707678147022981970025Zheng ZLiu YLi JZhang YZhang HGao JfalseIRAK-M has effects in regulation of lung epithelial inflammation.<h4>Background</h4>Epithelial barrier is important for asthma development by shaping immune responses. Airway expressing-IL-1 receptor-associated kinase (IRAK)-M of Toll-like receptor pathway was involved in immunoregulation of airway inflammation through influencing activities of macrophages and dendritic cells or T cell differentiation. Whether IRAK-M has effect on cellular immunity in airway epithelial cells upon stimulation remains unclear.<h4>Methods</h4>We modeled cellular inflammation induced by IL-1β, TNF-α, IL-33, and house dust mite (HDM) in BEAS-2B and A549 cells. Cytokine production and pathway activation were used to reflect the effects of IRAK-M siRNA knockdown on epithelial immunity. Genotyping an asthma-susceptible IRAK-M SNP rs1624395 and measurement of serum CXCL10 levels2023-01-01T00:00:00Z2023 Apr2026-05-29T01:09:50.219Z2024-11-15T20:01:52.192ZS-EPMC100825273702936310.1186/s12931-023-02406-5