{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14"],"submitter":["Wang L"],"pubmed_abstract":["We demonstrate two synthetic single-cell systems that can be used to better understand how the acquisition of an orphan gene can affect complex phenotypes. The Arabidopsis orphan gene, <i>Qua-Quine Starch</i> (<i>QQS</i>) has been identified as a regulator of carbon (C) and nitrogen (N) partitioning across multiple plant species. <i>QQS</i> modulates this important biotechnological trait by replacing NF-YB (Nuclear Factor Y, subunit B) in its interaction with NF-YC. In this study, we expand on these prior findings by developing <i>Chlamydomonas reinhardtii</i> and <i>Saccharomyces cerevisiae</i> strains, to refactor the functional interactions between QQS and NF-Y subunits to affect modulations in C and N allocation. Expression of <i>QQS</i> in <i>C. reinhardtii</i> modulates C (<i>i.e.</i"],"journal":["Frontiers in plant science"],"pagination":["1126139"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10084940"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation."],"pmcid":["PMC10084940"],"pubmed_authors":["Tonsager AJ","Zhang J","Zheng W","Meng Y","Li L","Wang L","Stenback KE","Tanvir R","Cothron S","Fang W","Campbell A","Spalding MH","Stessman D","Wang Y","Nikolau BJ","Wan D"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation.","description":"We demonstrate two synthetic single-cell systems that can be used to better understand how the acquisition of an orphan gene can affect complex phenotypes. The Arabidopsis orphan gene, <i>Qua-Quine Starch</i> (<i>QQS</i>) has been identified as a regulator of carbon (C) and nitrogen (N) partitioning across multiple plant species. <i>QQS</i> modulates this important biotechnological trait by replacing NF-YB (Nuclear Factor Y, subunit B) in its interaction with NF-YC. In this study, we expand on these prior findings by developing <i>Chlamydomonas reinhardtii</i> and <i>Saccharomyces cerevisiae</i> strains, to refactor the functional interactions between QQS and NF-Y subunits to affect modulations in C and N allocation. Expression of <i>QQS</i> in <i>C. reinhardtii</i> modulates C (<i>i.e.</i","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-22T05:03:45.991Z","creation":"2025-04-05T21:09:25.423Z"},"accession":"S-EPMC10084940","cross_references":{"pubmed":["37051080"],"doi":["10.3389/fpls.2023.1126139"]}}