{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pomari E"],"funding":["Italian Ministry of Health","EU funding within the MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases"],"pagination":["1349"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10093040"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(7)"],"pubmed_abstract":["<h4>Background</h4>Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family <i>Poxviridae</i>. Distinct clades are identified: the clade I belonging to the Central African (or Congo Basin) clade and the subclades IIa and IIb belonging to the West African clade. Here, a commercial droplet digital PCR (ddPCR) assay was evaluated for the quantification of the MPXV West Africa clade in clinical samples.<h4>Methods</h4>The ddPCR reaction was assessed as a duplex assay using <i>RPP30</i> as an internal amplification control. A total of 60 clinical specimens were tested, 40 positives (skin lesions, <i>n</i>=10; rectal swabs, <i>n</i> = 10; pharyngeal swabs, <i>n</i> = 10; and whole blood, <i>n</i> = 10), and 20 negatives (<i>n</i> = 5 for each biolo"],"journal":["Diagnostics (Basel, Switzerland)"],"pubmed_title":["Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples."],"pmcid":["PMC10093040"],"funding_grant_id":["\"Fondi Ricerca corrente - L1P3\"","Project no. PE00000007, INF-ACT"],"pubmed_authors":["Accordini S","Castilletti C","Pomari E","Donini A","Tacconelli E","Cordioli M","Mori A"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples.","description":"<h4>Background</h4>Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family <i>Poxviridae</i>. Distinct clades are identified: the clade I belonging to the Central African (or Congo Basin) clade and the subclades IIa and IIb belonging to the West African clade. Here, a commercial droplet digital PCR (ddPCR) assay was evaluated for the quantification of the MPXV West Africa clade in clinical samples.<h4>Methods</h4>The ddPCR reaction was assessed as a duplex assay using <i>RPP30</i> as an internal amplification control. A total of 60 clinical specimens were tested, 40 positives (skin lesions, <i>n</i>=10; rectal swabs, <i>n</i> = 10; pharyngeal swabs, <i>n</i> = 10; and whole blood, <i>n</i> = 10), and 20 negatives (<i>n</i> = 5 for each biolo","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-04T12:15:03.615Z","creation":"2025-04-04T12:15:03.615Z"},"accession":"S-EPMC10093040","cross_references":{"pubmed":["37046567"],"doi":["10.3390/diagnostics13071349"]}}