biostudies-literaturePomari EItalian Ministry of HealthEU funding within the MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases1349https://www.ebi.ac.uk/biostudies/studies/S-EPMC10093040biostudies-literatureUnknown13(7)<h4>Background</h4>Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family <i>Poxviridae</i>. Distinct clades are identified: the clade I belonging to the Central African (or Congo Basin) clade and the subclades IIa and IIb belonging to the West African clade. Here, a commercial droplet digital PCR (ddPCR) assay was evaluated for the quantification of the MPXV West Africa clade in clinical samples.<h4>Methods</h4>The ddPCR reaction was assessed as a duplex assay using <i>RPP30</i> as an internal amplification control. A total of 60 clinical specimens were tested, 40 positives (skin lesions, <i>n</i>=10; rectal swabs, <i>n</i> = 10; pharyngeal swabs, <i>n</i> = 10; and whole blood, <i>n</i> = 10), and 20 negatives (<i>n</i> = 5 for each bioloDiagnostics (Basel, Switzerland)Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples.PMC10093040"Fondi Ricerca corrente - L1P3"Project no. PE00000007, INF-ACTAccordini SCastilletti CPomari EDonini ATacconelli ECordioli MMori AfalseEvaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples.<h4>Background</h4>Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family <i>Poxviridae</i>. Distinct clades are identified: the clade I belonging to the Central African (or Congo Basin) clade and the subclades IIa and IIb belonging to the West African clade. Here, a commercial droplet digital PCR (ddPCR) assay was evaluated for the quantification of the MPXV West Africa clade in clinical samples.<h4>Methods</h4>The ddPCR reaction was assessed as a duplex assay using <i>RPP30</i> as an internal amplification control. A total of 60 clinical specimens were tested, 40 positives (skin lesions, <i>n</i>=10; rectal swabs, <i>n</i> = 10; pharyngeal swabs, <i>n</i> = 10; and whole blood, <i>n</i> = 10), and 20 negatives (<i>n</i> = 5 for each biolo2023-01-01T00:00:00Z2023 Apr2025-04-04T12:15:03.615Z2025-04-04T12:15:03.615ZS-EPMC100930403704656710.3390/diagnostics13071349