{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Peng CC"],"funding":["The Institute for Families, Inc.","The Larry and Celia Moh Foundation","Children's Hospital Los Angeles","Berle & Lucy Adams Chair in Cancer Research","Knights Templar Eye Foundation","NCI NIH HHS","The Dr. Miriam and Sheldon G. Adelson Medical Research Foundation"],"pagination":["6825"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10094875"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(7)"],"pubmed_abstract":["Gene expression profiling (GEP) is clinically validated to stratify the risk of metastasis by assigning uveal melanoma (UM) patients to two highly prognostic molecular classes: class 1 (low metastatic risk) and class 2 (high metastatic risk). However, GEP requires intraocular tumor biopsy, which is limited by small tumor size and tumor heterogeneity; furthermore, there are small risks of retinal hemorrhage, bleeding, or tumor dissemination. Thus, ocular liquid biopsy has emerged as a less-invasive alternative. In this study, we seek to determine the aqueous humor (AH) proteome related to the advanced GEP class 2 using diagnostic AH liquid biopsy specimens. Twenty AH samples were collected from patients with UM, grouped by GEP classes. Protein expression levels of 1472 targets were analyzed, compared between GEP classes, and correlated with clinical features. Significant differentially expressed proteins (DEPs) were subjected to analysis for cellular pathway and upstream regulator identification. The results showed that 45 DEPs detected in the AH could differentiate GEP class 1 and 2 at diagnosis. IL1R and SPRY2 are potential upstream regulators for the 8/45 DEPs that contribute to metastasis-related pathways. AH liquid biopsy offers a new opportunity to determine metastatic potential for patients in the absence of tumor biopsy."],"journal":["International journal of molecular sciences"],"pubmed_title":["Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma."],"pmcid":["PMC10094875"],"funding_grant_id":["The Institute for Families, Inc.","The Larry and Celia Moh Foundation","Research to Prevent Blindness","Berle & Lucy Adams Chair in Cancer Research","Knights Templar Eye Foundation","K08CA232344","The Dr. Miriam and Sheldon G. Adelson Medical Research Foundation"],"pubmed_authors":["Li HT","Xu L","Sirivolu S","Liang G","Berry JL","Reiser B","Peng CC","Pike S","Kim ME"],"additional_accession":[]},"is_claimable":false,"name":"Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma.","description":"Gene expression profiling (GEP) is clinically validated to stratify the risk of metastasis by assigning uveal melanoma (UM) patients to two highly prognostic molecular classes: class 1 (low metastatic risk) and class 2 (high metastatic risk). However, GEP requires intraocular tumor biopsy, which is limited by small tumor size and tumor heterogeneity; furthermore, there are small risks of retinal hemorrhage, bleeding, or tumor dissemination. Thus, ocular liquid biopsy has emerged as a less-invasive alternative. In this study, we seek to determine the aqueous humor (AH) proteome related to the advanced GEP class 2 using diagnostic AH liquid biopsy specimens. Twenty AH samples were collected from patients with UM, grouped by GEP classes. Protein expression levels of 1472 targets were analyzed, compared between GEP classes, and correlated with clinical features. Significant differentially expressed proteins (DEPs) were subjected to analysis for cellular pathway and upstream regulator identification. The results showed that 45 DEPs detected in the AH could differentiate GEP class 1 and 2 at diagnosis. IL1R and SPRY2 are potential upstream regulators for the 8/45 DEPs that contribute to metastasis-related pathways. AH liquid biopsy offers a new opportunity to determine metastatic potential for patients in the absence of tumor biopsy.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Apr","modification":"2025-04-04T12:15:43.426Z","creation":"2025-04-04T12:15:43.426Z"},"accession":"S-EPMC10094875","cross_references":{"pubmed":["37047796"],"doi":["10.3390/ijms24076825"]}}